Metagenomic 16S rDNA reads of<i> in</i><i> situ</i> preserved samples revealed microbial communities in the Yongle blue hole

doi:10.7717/peerj.16257

CORC > 深海科学与工程研究所 > 中国科学院深海科学与工程研究所 > 深海科学研究部 > 深海生物学研究室 > 深海生物蛋白质组学及分子生物学研究组

	Metagenomic 16S rDNA reads of in situ preserved samples revealed microbial communities in the Yongle blue hole
	Zhang, Hongxi 1,2; Wei, Taoshu 1; Li, Qingmei 1; Fu, Liang 3; He, Lisheng 1; Wang, Yong 4
刊名	PEERJ
	2023-11-03
卷号	11 页码:18
关键词	Yongle blue hole In situ sampling ISMIFF 16S rRNA gene Anoxic water
ISSN号	2167-8359
DOI	10.7717/peerj.16257
通讯作者	Wang, Yong(wangyong@sz.tsinghua.edu.cn)
英文摘要	Our knowledge on biogeochemistry and microbial ecology of marine blue holes is limited due to challenges in collecting multilayered water column and oxycline zones. In this study, we collected samples from 16 water layers in Yongle blue hole (YBH) located in the South China Sea using the in situ microbial filtration and fixation (ISMIFF) apparatus. The microbial communities based on 16S rRNA metagenomic reads for the ISMIFF samples showed high microbial diversity and consistency among samples with similar dissolved oxygen levels. At the same depth of the anoxic layer, the ISMIFF samples were dominated by sulfate-reducing bacteria from Desulfatiglandales (17.96%). The sulfide concentration is the most significant factor that drives the division of microbial communities in YBH, which might support the prevalence of sulfate-reducing microorganisms in the anoxic layers. Our results are different from the microbial community structures of a Niskin sample of this study and the reported samples collected in 2017, in which a high relative abundance of Alteromonadales (26.59%) and Thiomicrospirales (38.13%), and Arcobacteraceae (11.74%) was identified. We therefore demonstrate a new profile of microbial communities in YBH probably due to the effect of sampling and molecular biological methods, which provides new possibilities for further understanding of the material circulation mechanism of blue holes and expanding anoxic marine water zones under global warming.
WOS关键词	OXYGEN ; WATER ; NITRATE
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	PEERJ INC
WOS记录号	WOS:001101101800003
内容类型	期刊论文
源URL	[http://ir.idsse.ac.cn/handle/183446/10667]
专题	深海科学研究部_深海生物学研究室_深海生物蛋白质组学及分子生物学研究组
通讯作者	Wang, Yong
作者单位	1.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya, Hainan, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Sansha Trackline Inst Coral Reef Environm Protect, Sansha, Hainan, Peoples R China 4.Tsinghua Univ, Inst Ocean Engn, Shenzhen Int Grad Sch, Shenzhen, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Hongxi,Wei, Taoshu,Li, Qingmei,et al. Metagenomic 16S rDNA reads of in situ preserved samples revealed microbial communities in the Yongle blue hole[J]. PEERJ,2023,11:18.
APA	Zhang, Hongxi,Wei, Taoshu,Li, Qingmei,Fu, Liang,He, Lisheng,&Wang, Yong.(2023).Metagenomic 16S rDNA reads of in situ preserved samples revealed microbial communities in the Yongle blue hole.PEERJ,11,18.
MLA	Zhang, Hongxi,et al."Metagenomic 16S rDNA reads of in situ preserved samples revealed microbial communities in the Yongle blue hole".PEERJ 11(2023):18.