The TorRS two component system regulates expression of TMAO reductase in response to high hydrostatic pressure in Vibrio fluvialis

doi:10.3389/fmicb.2023.1291578

CORC > 深海科学与工程研究所 > 中国科学院深海科学与工程研究所 > 深海科学研究部 > 深海生物学研究室 > 深海微生物细胞生物学研究组

	The TorRS two component system regulates expression of TMAO reductase in response to high hydrostatic pressure in Vibrio fluvialis
	Liu, Na 1,2; Jiang, Ting 1,2; Cui, Wen-Peng 1,2; Qi, Xiao-Qing 1,3,4; Li, Xue-Gong 1,3,4; Lu, Yuan 5; Wu, Long-Fei 4,6; Zhang, Wei-Jia 1,3,4
刊名	FRONTIERS IN MICROBIOLOGY
	2023-11-07
卷号	14 页码:10
关键词	deep-sea high hydrostatic pressure adaptation TMAO reduction two-component system TorRS
DOI	10.3389/fmicb.2023.1291578
通讯作者	Wu, Long-Fei(wzhang@idsse.ac.cn) ; Zhang, Wei-Jia(wu@imm.cnrs.fr)
英文摘要	High hydrostatic pressure (HHP) regulated gene expression is one of the most commonly adopted strategies for microbial adaptation to the deep-sea environments. Previously we showed that the HHP-inducible trimethylamine N-oxide (TMAO) reductase improves the pressure tolerance of deep-sea strain Vibrio fluvialis QY27. Here, we investigated the molecular mechanism of HHP-responsive regulation of TMAO reductase TorA. By constructing torR and torS deletion mutants, we demonstrated that the two-component regulator TorR and sensor TorS are responsible for the HHP-responsive regulation of torA. Unlike known HHP-responsive regulatory system, the abundance of torR and torS was not affected by HHP. Complementation of the Delta torS mutant with TorS altered at conserved phosphorylation sites revealed that the three sites were indispensable for substrate-induced regulation, but only the histidine located in the alternative transmitter domain was involved in pressure-responsive regulation. Taken together, we demonstrated that the induction of TMAO reductase by HHP is mediated through the TorRS system and proposed a bifurcation of signal transduction in pressure-responsive regulation from the substrate-induction. This work provides novel knowledge of the pressure regulated gene expression and will promote the understanding of the microbial adaptation to the deep-sea HHP environment.
资助项目	The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This work was supported by grant NSFC91751108, 42076127 and 42176121 from the National Natural Science Foundation of China, grant ZDKJ202[NSFC91751108] ; The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This work was supported by grant NSFC91751108, 42076127 and 42176121 from the National Natural Science Foundation of China, grant ZDKJ202[42076127] ; The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This work was supported by grant NSFC91751108, 42076127 and 42176121 from the National Natural Science Foundation of China, grant ZDKJ202[42176121] ; National Natural Science Foundation of China[ZDKJ2021028] ; National Natural Science Foundation of China[ZDKJ2019011] ; Key Research and Development Program of Hainan Province[XDA19060403] ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Chinese Academy of Sciences
WOS关键词	TRIMETHYLAMINE-N-OXIDE ; DEEP-SEA BACTERIUM ; ESCHERICHIA-COLI ; UNORTHODOX SENSOR ; GENE-EXPRESSION ; BINDING ; OPERON ; INDUCTION ; STRESS
WOS研究方向	Microbiology
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:001106312700001
资助机构	The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This work was supported by grant NSFC91751108, 42076127 and 42176121 from the National Natural Science Foundation of China, grant ZDKJ202 ; National Natural Science Foundation of China ; Key Research and Development Program of Hainan Province ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Chinese Academy of Sciences
内容类型	期刊论文
源URL	[http://ir.idsse.ac.cn/handle/183446/10676]
专题	深海科学研究部_深海生物学研究室_深海微生物细胞生物学研究组
通讯作者	Wu, Long-Fei; Zhang, Wei-Jia
作者单位	1.Chinese Acad Sci, Lab Deep Sea Microbial Cell Biol, Inst Deep Sea Sci & Engn, Sanya, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Inst Deep Sea Life Sci, IDSSE BGI, Sanya, Peoples R China 4.Chinese Acad Sci, Int Associated Lab Evolut & Dev Magnetotact Multic, Sanya, Peoples R China 5.Ocean Univ China, Coll Informat Sci & Engn, Qingdao, Peoples R China 6.Aix Marseille Univ, CNRS, LCB, Marseille, France
推荐引用方式 GB/T 7714	Liu, Na,Jiang, Ting,Cui, Wen-Peng,et al. The TorRS two component system regulates expression of TMAO reductase in response to high hydrostatic pressure in Vibrio fluvialis[J]. FRONTIERS IN MICROBIOLOGY,2023,14:10.
APA	Liu, Na.,Jiang, Ting.,Cui, Wen-Peng.,Qi, Xiao-Qing.,Li, Xue-Gong.,...&Zhang, Wei-Jia.(2023).The TorRS two component system regulates expression of TMAO reductase in response to high hydrostatic pressure in Vibrio fluvialis.FRONTIERS IN MICROBIOLOGY,14,10.
MLA	Liu, Na,et al."The TorRS two component system regulates expression of TMAO reductase in response to high hydrostatic pressure in Vibrio fluvialis".FRONTIERS IN MICROBIOLOGY 14(2023):10.