Comparison of analytical sensitivity and efficiency for SARS-CoV-2 primer sets by TaqMan-based and SYBR Green-based RT-qPCR

doi:10.1007/s00253-022-11822-4

CORC > 地球环境研究所 > 中国科学院地球环境研究所 > 粉尘与环境研究室

	Comparison of analytical sensitivity and efficiency for SARS-CoV-2 primer sets by TaqMan-based and SYBR Green-based RT-qPCR
	Tao, Yile 8,9; Yue, Yang 8,9; Qiu, Guangyu 8,9; Ji, Zheng 7; Spillman, Martin 8,9; Gai, Zhibo 6; Chen, Qingfa 5; Bielecki, Michel 4; Huber, Michael 3; Trkola, Alexandra 3
刊名	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
	2022-02-26
页码	12
关键词	SARS-CoV-2 COVID-19 SYBR Green TaqMan probe RT-qPCR
ISSN号	0175-7598
DOI	10.1007/s00253-022-11822-4
通讯作者	Wang, Jing(jing.wang@ifu.baug.ethz.ch)
英文摘要	The pandemic of coronavirus disease 2019 (COVID-19) continues to threaten public health. For developing countries where vaccines are still in shortage, cheaper alternative molecular methods for SARS-CoV-2 identification can be crucial to prevent the next wave. Therefore, 14 primer sets recommended by the World Health Organization (WHO) was evaluated on testing both clinical patient and environmental samples with the gold standard diagnosis method, TaqMan-based RT-qPCR, and a cheaper alternative method, SYBR Green-based RT-qPCR. Using suitable primer sets, such as ORF1ab, 2019_nCoV_N1 and 2019_nCoV_N3, the performance of the SYBR Green approach was comparable or better than the TaqMan approach, even when considering the newly dominating or emerging variants, including Delta, Eta, Kappa, Lambda, Mu, and Omicron. ORF1ab and 2019_nCoV_N3 were the best combination for sensitive and reliable SARS-CoV-2 molecular diagnostics due to their high sensitivity, specificity, and broad accessibility.
资助项目	China Scholarship Council[201906875037] ; China Scholarship Council[201706010410]
WOS关键词	POLYMERASE-CHAIN-REACTION ; COVID-19 ; PCR
WOS研究方向	Biotechnology & Applied Microbiology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000761867000005
资助机构	China Scholarship Council
内容类型	期刊论文
源URL	[http://ir.ieecas.cn/handle/361006/17472]
专题	地球环境研究所_粉尘与环境研究室
通讯作者	Wang, Jing
作者单位	1.CAS Ctr Excellence Quaternary Sci & Global Change, Xian 710061, Peoples R China 2.Chinese Acad Sci, Inst Earth Environm, Key Lab Aerosol Chem & Phys, State Key Lab Loess & Quaternary Geol, Xian 710061, Peoples R China 3.Univ Zurich, Inst Med Virol, CH-8057 Zurich, Switzerland 4.Univ Zurich, Epidemiol Biostat & Prevent Inst, CH-8091 Zurich, Switzerland 5.Liaocheng Univ, Inst Tissue Engn & Regenerat Med, Liaocheng 252000, Shandong, Peoples R China 6.Univ Zurich, Univ Hosp Zurich, Dept Clin Pharmacol & Toxicol, CH-8091 Zurich, Switzerland 7.Shaanxi Normal Univ, Sch Geog & Tourism, Xian 710119, Peoples R China 8.Swiss Fed Labs Mat Sci & Technol, Lab Adv Analyt Technol, Empa, CH-8600 Dubendorf, Switzerland 9.Swiss Fed Inst Technol, Inst Environm Engn, CH-8093 Zurich, Switzerland
推荐引用方式 GB/T 7714	Tao, Yile,Yue, Yang,Qiu, Guangyu,et al. Comparison of analytical sensitivity and efficiency for SARS-CoV-2 primer sets by TaqMan-based and SYBR Green-based RT-qPCR[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2022:12.
APA	Tao, Yile.,Yue, Yang.,Qiu, Guangyu.,Ji, Zheng.,Spillman, Martin.,...&Wang, Jing.(2022).Comparison of analytical sensitivity and efficiency for SARS-CoV-2 primer sets by TaqMan-based and SYBR Green-based RT-qPCR.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,12.
MLA	Tao, Yile,et al."Comparison of analytical sensitivity and efficiency for SARS-CoV-2 primer sets by TaqMan-based and SYBR Green-based RT-qPCR".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2022):12.