Membrane-Mediated Cooperative Interactions of CD47 and SIRP<i>&alpha;</i>

doi:10.3390/membranes13110871

CORC > 力学研究所 > 中国科学院力学研究所 > 非线性力学国家重点实验室

	Membrane-Mediated Cooperative Interactions of CD47 and SIRPα
	Li, Long 2,3,4; Gui, Chen 4; Hu, Jinglei 4; Rozycki, Bartosz 1
刊名	MEMBRANES
	2023-11-01
卷号	13 期号:11 页码:13
关键词	membrane adhesion biomimetics computer simulations CD47 SIRP alpha
DOI	10.3390/membranes13110871
通讯作者	Rozycki, Bartosz(rozycki@ifpan.edu.pl)
英文摘要	The specific binding of the ubiquitous 'marker of self' protein CD47 to the SIRP alpha protein anchored in the macrophage plasma membrane results in the inhibition of the engulfment of 'self' cells by macrophages and thus constitutes a key checkpoint of our innate immune system. Consequently, the CD47-SIRP alpha protein complex has been recognized as a potential therapeutic target in cancer and inflammation. Here, we introduce a lattice-based mesoscale model for the biomimetic system studied recently in fluorescence microscopy experiments where GFP-tagged CD47 proteins on giant plasma membrane vesicles bind to SIRP alpha proteins immobilized on a surface. Computer simulations of the lattice-based mesoscale model allow us to study the biomimetic system on multiple length scales, ranging from single nanometers to several micrometers and simultaneously keep track of single CD47-SIRP alpha binding and unbinding events. Our simulations not only reproduce data from the fluorescence microscopy experiments but also are consistent with results of several other experiments, which validates our numerical approach. In addition, our simulations yield quantitative predictions on the magnitude and range of effective, membrane-mediated attraction between CD47-SIRP alpha complexes. Such detailed information on CD47-SIRP alpha interactions cannot be obtained currently from experiments alone. Our simulation results thus extend the present understanding of cooperative effects in CD47-SIRP alpha interactions and may have an influence on the advancement of new cancer treatments.
资助项目	National Science Centre of Poland[2021/40/Q/NZ1/00017] ; National Natural Science Foundation of China[12232019] ; National Natural Science Foundation of China[12272388] ; National Natural Science Foundation of China[21973040] ; National Natural Science Foundation of China[22161132012] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences
WOS关键词	RECEPTOR-LIGAND BINDING ; ANCHORED RECEPTORS ; AGGREGATION ; ADHESION ; PROTEINS ; ENHANCE
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Engineering ; Materials Science ; Polymer Science
语种	英语
WOS记录号	WOS:001114541400001
资助机构	National Science Centre of Poland ; National Natural Science Foundation of China ; Youth Innovation Promotion Association of the Chinese Academy of Sciences
内容类型	期刊论文
源URL	[http://dspace.imech.ac.cn/handle/311007/93557]
专题	力学研究所_非线性力学国家重点实验室
通讯作者	Rozycki, Bartosz
作者单位	1.Polish Acad Sci, Inst Phys, Aleja Lotnikow 32-46, PL-02668 Warsaw, Poland 2.Chinese Acad Sci, Inst Mech, Beijing Key Lab Engn Construction & Mechanobiol, Beijing 100190, Peoples R China 3.Chinese Acad Sci, State Key Lab Nonlinear Mech, Beijing 100190, Peoples R China 4.Nanjing Univ, Kuang Yaming Honors Sch, Nanjing 210023, Peoples R China
推荐引用方式 GB/T 7714	Li, Long,Gui, Chen,Hu, Jinglei,et al. Membrane-Mediated Cooperative Interactions of CD47 and SIRPα[J]. MEMBRANES,2023,13(11):13.
APA	Li, Long,Gui, Chen,Hu, Jinglei,&Rozycki, Bartosz.(2023).Membrane-Mediated Cooperative Interactions of CD47 and SIRPα.MEMBRANES,13(11),13.
MLA	Li, Long,et al."Membrane-Mediated Cooperative Interactions of CD47 and SIRPα".MEMBRANES 13.11(2023):13.