Active pathways of anaerobic methane oxidization in deep-sea cold seeps of the South China Sea

doi:10.1128/spectrum.02505-23

CORC > 深海科学与工程研究所 > 中国科学院深海科学与工程研究所 > 深海科学研究部 > 深海生物学研究室 > 海洋微生物分子生态学研究组

	Active pathways of anaerobic methane oxidization in deep-sea cold seeps of the South China Sea
	Jiang, Qiuyun 4,5; Jing, Hongmei 2,3,5; Li, Xuegong 5; Wan, Ye 5; Chou, I-Ming 5; Hou, Lijun 1; Dong, Hongpo 1; Niu, Yuhui 1; Gao, Dengzhou 1
刊名	MICROBIOLOGY SPECTRUM
	2023-11-02
页码	18
关键词	cold seeps anaerobic methane oxidation isotopic tracing experiment electron acceptors (CH4)-C-13
ISSN号	2165-0497
DOI	10.1128/spectrum.02505-23
通讯作者	Jing, Hongmei(hmjing@idsse.ac.cn)
英文摘要	Anaerobic oxidation of methane (AOM) is critical for controlling methane emissions from deep-sea cold seeps. Microbial groups associated with different AOM processes have been briefly investigated, but the AOM activities associated with the utilization of different electron acceptors in the deep-sea methane seeps remain largely unknown. Here, surface sediments (0-4 cm) from two cold seeps (Site F/Haima) and the Xisha trough in the South China Sea (SCS) were incubated to measure AOM potentials driven by nitrite, nitrate, and sulfate as different electron acceptors with isotopically labeled methane gas ((CH4)-C-13), and the population shifts of microbial communities along the incubation were investigated by high-throughput sequencing and quantitative polymerase chain reaction based on both the 16S rRNA gene and functional genes. Nitrite and nitrate were preferentially used prior to sulfate during the incubation, and nitrate-/nitrite-dependent anaerobic methane oxidation (Nr-/N-DAMO) contributed more to the methane flux in both the cold seeps and the trough. The highest rates of DAMO and sulfate-dependent anaerobic methane oxidation (SAMO) were detected at Site F, consistent with the transcript abundance of mcrA and dsrB genes during the incubation, and might be explained by the in situ active functional groups. The former process might be explained by Campylobacteria, which could couple sulfide oxidation and nitrate consumption to further participate in the N-DAMO process, while the latter process might be due to the higher proportions of Desulfobacterota. This study elucidates the diversity and potential activities of major microbial groups in the DAMO and SAMO processes in the deep-sea cold seeps using isotopic tracing incubations and provides direct evidence for the occurrence of Nr-/N-DAMO as a previously overlooked microbial methane sink in the deep-sea hydrate-bearing sediments in the SCS.
资助项目	Q.J.: Conceptualization, Methodology, and Writing-original draft. H.J.: Conceptualization, Supervision, Writing-review and editing, and Funding acquisition. X.L.: Methodology and Writing-review and editing. Y.W.: Methodology and Writing-review and editing.
WOS关键词	16S RIBOSOMAL-RNA ; SULFATE-REDUCING BACTERIA ; COMMUNITY COMPOSITION ; ARCHAEAL COMMUNITY ; OXIDATION BACTERIA ; DIVERSITY ; SEDIMENTS ; NITRITE ; NITRATE ; CARBON
WOS研究方向	Microbiology
语种	英语
出版者	AMER SOC MICROBIOLOGY
WOS记录号	WOS:001099052600003
资助机构	Q.J.: Conceptualization, Methodology, and Writing-original draft. H.J.: Conceptualization, Supervision, Writing-review and editing, and Funding acquisition. X.L.: Methodology and Writing-review and editing. Y.W.: Methodology and Writing-review and editing.
内容类型	期刊论文
源URL	[http://ir.idsse.ac.cn/handle/183446/10652]
专题	深海科学研究部_深海生物学研究室_海洋微生物分子生态学研究组
通讯作者	Jing, Hongmei
作者单位	1.East China Normal Univ, State Key Lab Estuarine & Costal Res, Shanghai, Peoples R China 2.Chinese Acad Sci, HKUST CAS Sanya Joint Lab Marine Sci Res, Sanya, Peoples R China 3.Southern Marine Sci & Engn Guangdong Lab, Zhuhai, Guangdong, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China 5.Chinese Acad Sci, Inst Deep Sea Sci & Engn, CAS Key Lab Expt Study Deep Sea Extreme Condit, Sanya, Peoples R China
推荐引用方式 GB/T 7714	Jiang, Qiuyun,Jing, Hongmei,Li, Xuegong,et al. Active pathways of anaerobic methane oxidization in deep-sea cold seeps of the South China Sea[J]. MICROBIOLOGY SPECTRUM,2023:18.
APA	Jiang, Qiuyun.,Jing, Hongmei.,Li, Xuegong.,Wan, Ye.,Chou, I-Ming.,...&Gao, Dengzhou.(2023).Active pathways of anaerobic methane oxidization in deep-sea cold seeps of the South China Sea.MICROBIOLOGY SPECTRUM,18.
MLA	Jiang, Qiuyun,et al."Active pathways of anaerobic methane oxidization in deep-sea cold seeps of the South China Sea".MICROBIOLOGY SPECTRUM (2023):18.