TorRS two component system regulates expression of TMAO reductase in response to high hydrostatic pressure through a distinctive signal transduction pathway | |
Na Liu; Ting Jiang; Wen-Peng Cui; Xiao-Qing Qi; Xue-Gong Li; Yuan Lu; Long-Fei Wu; Wei-Jia Zhang | |
刊名 | Frontiers In Microbiology |
2023-11-07 | |
卷号 | 14页码:1 |
关键词 | TMAO reduction TorRS adaptation deep-sea high hydrostatic pressure two-component system. |
DOI | 10.3389/fmicb.2023.1291578 |
通讯作者 | Long-Fei Wu ; Wei-Jia Zhang |
英文摘要 | High hydrostatic pressure (HHP) regulated gene expression is one of the most commonly adopted strategies for microbial adaptation to the deep-sea environments. Previously we showed that the HHP-inducible trimethylamine N-oxide (TMAO) reductase improves the pressure tolerance of deep-sea strain Vibrio fluvialis QY27. Here, we investigated the molecular mechanism of HHP responsive regulation of TMAO reductase TorA. By constructing torR and torS deletion mutants, we demonstrated that the two-component regulator TorR and sensor TorS are responsible for the HHP-responsive regulation of torA. Unlike known HHP-responsive regulatory system, the abundance of torR and torS was not affected by HHP. Complementation of the ΔtorS mutant with TorS altered at conserved phosphorylation sites revealed that the three sites were indispensable for substrate-induced regulation, but only the histidine located in the alternative transmitter domain was involved in pressure-responsive regulation. Taken together, we demonstrated that the induction of TMAO reductase by HHP is mediated through the TorRS system and proposed a bifurcation of signal transduction in pressure-responsive regulation from the substrate-induction. This work provides novel knowledge of the pressure regulated gene expression and will promote the understanding of the microbial adaptation to the deep-sea HHP environment. |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://ir.idsse.ac.cn/handle/183446/10647] |
专题 | 深海科学研究部_深海生物学研究室_深海微生物细胞生物学研究组 |
通讯作者 | Long-Fei Wu; Wei-Jia Zhang |
作者单位 | 中国科学院深海科学与工程研究所 |
推荐引用方式 GB/T 7714 | Na Liu,Ting Jiang,Wen-Peng Cui,et al. TorRS two component system regulates expression of TMAO reductase in response to high hydrostatic pressure through a distinctive signal transduction pathway[J]. Frontiers In Microbiology,2023,14:1. |
APA | Na Liu.,Ting Jiang.,Wen-Peng Cui.,Xiao-Qing Qi.,Xue-Gong Li.,...&Wei-Jia Zhang.(2023).TorRS two component system regulates expression of TMAO reductase in response to high hydrostatic pressure through a distinctive signal transduction pathway.Frontiers In Microbiology,14,1. |
MLA | Na Liu,et al."TorRS two component system regulates expression of TMAO reductase in response to high hydrostatic pressure through a distinctive signal transduction pathway".Frontiers In Microbiology 14(2023):1. |
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