Screening and characterization of inhibitory vNAR targeting nanodisc-assembled influenza M2 proteins

doi:10.1016/j.isci.2022.105736

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Screening and characterization of inhibitory vNAR targeting nanodisc-assembled influenza M2 proteins
	Yu, Chuandi 1,9; Ding, Wen 8; Zhu, Lei ; Zhou, Yuhang 1,7; Dong, Yingkui 1,6; Li, Ling 1; Liu, Juanjuan ; Wang, Yizhuo 9; Li, Zehua 9; Zhu, Lina 9
刊名	ISCIENCE
	2023-01-20
卷号	26 期号:1 页码:21
DOI	10.1016/j.isci.2022.105736
通讯作者	Zha, Lisha(zhalisha@ahau.edu.cn) ; Zhao, Hongxin(zhx@hmfl.ac.cn) ; Wang, Junfeng(junfeng@hmfl.ac.cn)
英文摘要	Influenza A virus poses a constant challenge to human health. The highly conserved influenza matrix-2 (M2) protein is an attractive target for the development of a universal antibody-based drug. However, screening using antigens with subphysiological conformation in a nonmembrane environment significantly reduces the generation of efficient antibodies. Here, M2(1-46) was incorporated into nanodiscs (M2-nanodiscs) with M2 in a membrane-embedded tetrameric conformation, closely resembling its natural physiological state in the influenza viral envelope. M2-nanodisc generation, an antigen, was followed by Chiloscyllium plagiosum immunization. The functional vNARs were selected by phage display panning strategy from the shark immune library. One of the isolated vNARs, AM2H10, could specifically bind to tetrameric M2 instead of monomeric M2e (the ectodomain of M2 protein). Furthermore, AM2H10 blocked ion influx through amantadine-sensitive and resistant M2 channels. Our findings indicated the possibility of developing functional shark nanobodies against various influenza viruses by targeting the M2 protein.
资助项目	Anhui Technologies Major Program[201903a07020026] ; Chinese Academy of Sciences[YZJJZX202004] ; Users with Excellence Program of Hefei Science Center,CAS[2020HSC-UE018] ; National Natural Science Foundation of China[41876168] ; Taishan Young Scholar Program of Shandong Province[tsqn201812108]
WOS关键词	INTEGRAL MEMBRANE-PROTEINS ; SINGLE-DOMAIN ; MONOCLONAL-ANTIBODY ; LIPID-BILAYERS ; A VIRUSES ; V-REGION ; SHARK ; CHANNEL ; GENERATION ; SELECTION
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	CELL PRESS
WOS记录号	WOS:000901534700006
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/183260]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Zha, Lisha; Zhao, Hongxin; Wang, Junfeng
作者单位	1.Chinese Acad Sci, Hefei Inst Phys Sci, High Magnet Field Lab, Key Lab High Magnet Field & Ion Beam Phys Biol, Hefei 230031, Anhui, Peoples R China 2.Chinese Acad Sci, Shanghai Adv Res Inst, Natl Facil Prot Sci Shanghai, Shanghai 201210, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol Qingdao, Marine Biol & Biotechnol Lab, Qingdao 266237, Shandong, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Ctr OceanMega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 5.Hefei China Sci Longwood Biol Technol Co Ltd, Hefei 230088, Anhui, Peoples R China 6.Anhui Univ, Inst Phys Sci & Informat Technol, Hefei 230039, Anhui, Peoples R China 7.Anhui Agr Univ, Int Immunol Ctr, Hefei 230036, Anhui, Peoples R China 8.Anhui Med Univ, Sch Basic Med Sci, Hefei 230032, Anhui, Peoples R China 9.Univ Sci & Technol China, Hefei 230026, Anhui, Peoples R China
推荐引用方式 GB/T 7714	Yu, Chuandi,Ding, Wen,Zhu, Lei,et al. Screening and characterization of inhibitory vNAR targeting nanodisc-assembled influenza M2 proteins[J]. ISCIENCE,2023,26(1):21.
APA	Yu, Chuandi.,Ding, Wen.,Zhu, Lei.,Zhou, Yuhang.,Dong, Yingkui.,...&Wang, Junfeng.(2023).Screening and characterization of inhibitory vNAR targeting nanodisc-assembled influenza M2 proteins.ISCIENCE,26(1),21.
MLA	Yu, Chuandi,et al."Screening and characterization of inhibitory vNAR targeting nanodisc-assembled influenza M2 proteins".ISCIENCE 26.1(2023):21.