Effects of different dehydration methods on the preservation of aortic and renal glycocalyx structures in mice | |
Li, Zhi2,3,4; Zhang, Quanbin2,3; Sun, Yuan-yuan2; Wu, Ning1,2,3,5 | |
刊名 | HELIYON |
2023-04-01 | |
卷号 | 9期号:4页码:9 |
关键词 | Glycocalyx Dehydration Transmission electron microscopy |
DOI | 10.1016/j.heliyon.2023.e15197 |
通讯作者 | Sun, Yuan-yuan(sunyuanyuan@qdio.ac.cn) ; Wu, Ning(wuning@qdio.ac.cn) |
英文摘要 | Glycocalyx is located outside the vascular endothelial cells playing an important role in vascular homeostasis. However, lacking efficient detection methods is one of the biggest obstacles to study the glycocalyx. In this study, three dehydration methods were used to compare the preservation of HUVEC, aorta and kidney glycocalyx by transmission electron microscope. The chemical pre -fixation was performed by lanthanum nitrate staining, and the mice aorta and renal glycocalyx were prepared by different dehydration methods such as ethanol gradient, acetone gradient and low temperature dehydration. HUVEC glycocalyx was prepared by acetone gradient and low temperature dehydration. Low temperature dehydration method preserves HUVEC and mice aortic glycocalyx completely, which had a certain thickness and presented a needle-like structure. But for mice kidney, the acetone gradient dehydration preparation method could better preserve the glycocalyx integrity than other two methods. In conclusion, low temperature dehydration method is suitable for HUVEC and aortic glycocalyx preservation, acetone gradient dehydration method is more suitable for kidney glycocalyx preservation. |
资助项目 | Innovative Research Group Project of the National Natural Science Foundation of China[42176137] ; Nantong Science and Technology Project, China[MS12021037] |
WOS关键词 | ENDOTHELIAL GLYCOCALYX ; DYSFUNCTION ; CELL ; SULFATE ; BIOLOGY ; LAYER |
WOS研究方向 | Science & Technology - Other Topics |
语种 | 英语 |
出版者 | CELL PRESS |
WOS记录号 | WOS:000999832300001 |
内容类型 | 期刊论文 |
源URL | [http://ir.qdio.ac.cn/handle/337002/183165] |
专题 | 海洋研究所_实验海洋生物学重点实验室 |
通讯作者 | Sun, Yuan-yuan; Wu, Ning |
作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Dept Drugs & Bioprod, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China 5.Nantong Zhongke Marine Sci & Technol Res & Dev Ctr, Nantong, Peoples R China |
推荐引用方式 GB/T 7714 | Li, Zhi,Zhang, Quanbin,Sun, Yuan-yuan,et al. Effects of different dehydration methods on the preservation of aortic and renal glycocalyx structures in mice[J]. HELIYON,2023,9(4):9. |
APA | Li, Zhi,Zhang, Quanbin,Sun, Yuan-yuan,&Wu, Ning.(2023).Effects of different dehydration methods on the preservation of aortic and renal glycocalyx structures in mice.HELIYON,9(4),9. |
MLA | Li, Zhi,et al."Effects of different dehydration methods on the preservation of aortic and renal glycocalyx structures in mice".HELIYON 9.4(2023):9. |
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