Establishment and application of four long-term culture cell lines of the olive flounder Paralichthys olivaceus blastocysts

doi:10.1016/j.cbpc.2022.109536

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Establishment and application of four long-term culture cell lines of the olive flounder Paralichthys olivaceus blastocysts
	Nie, Miaomiao 1,2,5; Zou, Congcong 2,4,5; Peng, Limin 3; Wu, Zhihao 2,4,5; You, Feng 2,4,5
刊名	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
	2023-03-01
卷号	265 页码:13
关键词	Flounder Embryo Cell line Long-term culture Toxicological study
ISSN号	1532-0456
DOI	10.1016/j.cbpc.2022.109536
通讯作者	You, Feng(youfeng@qdio.ac.cn)
英文摘要	Four new embryonic cell lines derived from blastocysts of the olive flounder Paralichthys olivaceus, an important commercial marine fish, were established and characterized. They were designated as PoEFCI, PoEFCII, PoEF-CIII, and PoEFCIV and were all fibroblastic cells. The cells were cultured in DMEM/F-12 medium supplemented with antibiotics, FBS, and growth factors at temperature of 25 degrees C and subcultured for >100 passages over 18 months. The origin of the cell lines was confirmed by examining the partial sequences of the cytochrome oxidase c subunit I (COI) gene of the flounder mitochondrial DNA (mtDNA). The four cell lines showed different growth curve patterns. According to the results of gene and protein expression and enzyme activity, the cell lines PoEFCI, PoEFCII, and PoEFC III could be pluripotent. The cells of all four cell lines were also successfully transfected with the green fluorescent protein (GFP) reporter gene, suggesting that they could be used to study gene function in the flounder or other fish. More importantly, PoEFCI-III were sensitive to chromium (Cr) and red sea bream Pagrus major iridovirus (RSIV), so they could be used as a powerful tool for the study of the toxicological investigation of heavy metals and RSIV in fish. Therefore, these cell lines would be useful for biotechnological and toxicological research on marine fish as an in vitro biological system.
资助项目	National Key Research and Development Program of China[2018YFD0900202] ; National Key Research and Development Program of China[2022YFD2400402] ; National Natural Science Foundation of China[31772834] ; Key Research and Development Programs of Shandong Province[2021LZGC029] ; National Marine Genetic Resource Center ; Innovation capability improvement project of TSMEs of Shandong Province[2021TSGC1234]
WOS关键词	LYMPHOCYSTIS DISEASE VIRUS ; BREAM IRIDOVIRAL DISEASE ; JAPANESE FLOUNDER ; SEA BREAM ; INTERMEDIATE-FILAMENTS ; GROWTH-RESPONSES ; FISH ; CYTOTOXICITY ; DERIVATION ; TOXICITY
WOS研究方向	Biochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology
语种	英语
出版者	ELSEVIER SCIENCE INC
WOS记录号	WOS:000995308400001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/183065]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	You, Feng
作者单位	1.Qinghai Univ, State Key Lab Plateau Ecol & Agr, 251 Ningda Rd, Xining 810016, Peoples R China 2.Chinese Acad Sci, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Qingdao 266071, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 5.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Shandong Prov Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Nie, Miaomiao,Zou, Congcong,Peng, Limin,et al. Establishment and application of four long-term culture cell lines of the olive flounder Paralichthys olivaceus blastocysts[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,2023,265:13.
APA	Nie, Miaomiao,Zou, Congcong,Peng, Limin,Wu, Zhihao,&You, Feng.(2023).Establishment and application of four long-term culture cell lines of the olive flounder Paralichthys olivaceus blastocysts.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,265,13.
MLA	Nie, Miaomiao,et al."Establishment and application of four long-term culture cell lines of the olive flounder Paralichthys olivaceus blastocysts".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY 265(2023):13.