Therapeutic potential of enzymatically extracted eumelanin from squid ink in type 2 diabetes mellitus ICR mice: Multifaceted intervention against hyperglycemia, oxidative stress and depression

doi:10.1002/jsfa.12986

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Therapeutic potential of enzymatically extracted eumelanin from squid ink in type 2 diabetes mellitus ICR mice: Multifaceted intervention against hyperglycemia, oxidative stress and depression
	Song, Wen 1,2,3,4; Xing, Ronge 2,3,4,5,6; Yang, Haoyue 3,4; Liu, Song 2,3,4; Yu, Huahua 2,3,4; Li, Pengcheng 2,3,4
刊名	JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
	2023-09-29
页码	15
关键词	type 2 diabetes mellitus (T2DM) eumelanin hypoglycemic activity antioxidant activity antidepressant activity
ISSN号	0022-5142
DOI	10.1002/jsfa.12986
通讯作者	Xing, Ronge(xingronge@qdio.ac.cn)
英文摘要	Background: Type 2 diabetes mellitus (T2DM) is a prevalent metabolic disease that poses significant health risks due to its numerous complications. However, the effects of eumelanin on oxidative stress, hyperglycemia and depression in diabetic mice have not been extensively studied. Results: Our study employed an enzymatic approach to extract eumelanin from squid ink and characterized it using spectroscopic techniques. Remarkably, eumelanin extracted with alkaline-neutral-flavor protease (ANF) displayed superior inhibitory activity against alpha-glucosidase and alpha-amylase, while enhancing glucose utilization and hepatic glycogen synthesis in human hepatocellular carcinoma cell line (HepG2) insulin resistance model. Further evaluation of ANF in a T2DM ICR mouse model demonstrated its significant potential in alleviating hyperglycemia, reducing glycosylated serum protein levels, improving glucose tolerance and modulating total cholesterol and low-density lipoprotein levels, as well as antioxidant indices at a dosage of 0.04 g kg(-1). Additionally, ANF exhibited positive effects on energy levels and reduced immobility time in antidepressant behavioral experiments. Moreover, ANF positively influenced the density and infiltration state of renal cells, while mitigating inflammatory enlargement and deformation of liver cells, without inducing any adverse effects in mice. Conclusion: Overall, these findings underscore the significant therapeutic potential of ANF in the treatment of T2DM and its associated complications. By augmenting lipid and glucose metabolism, mitigating oxidative stress and alleviating depression, ANF emerges as a promising candidate for multifaceted intervention. (c) 2023 Society of Chemical Industry.
资助项目	14th Five-Year Plan of Qingdao Pilot National Laboratory of Marine Science and Technology[2022QNLM03003-2] ; Shandong Key RD Plan ; Major Scientific and Technological Innovation Project[2022CXGC020413] ; Fujian Science and Technology Planning Project-STS Program[2021 T3013]
WOS关键词	ALPHA-GLUCOSIDASE ; MELANIN ; MODEL ; AMYLASE
WOS研究方向	Agriculture ; Chemistry ; Food Science & Technology
语种	英语
出版者	WILEY
WOS记录号	WOS:001075206100001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/181703]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Xing, Ronge
作者单位	1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Drugs & Bioprod, Qingdao, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 4.Chinese Acad Sci, Qingdao, Peoples R China 5.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 6.Chinese Acad Sci, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Song, Wen,Xing, Ronge,Yang, Haoyue,et al. Therapeutic potential of enzymatically extracted eumelanin from squid ink in type 2 diabetes mellitus ICR mice: Multifaceted intervention against hyperglycemia, oxidative stress and depression[J]. JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE,2023:15.
APA	Song, Wen,Xing, Ronge,Yang, Haoyue,Liu, Song,Yu, Huahua,&Li, Pengcheng.(2023).Therapeutic potential of enzymatically extracted eumelanin from squid ink in type 2 diabetes mellitus ICR mice: Multifaceted intervention against hyperglycemia, oxidative stress and depression.JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE,15.
MLA	Song, Wen,et al."Therapeutic potential of enzymatically extracted eumelanin from squid ink in type 2 diabetes mellitus ICR mice: Multifaceted intervention against hyperglycemia, oxidative stress and depression".JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE (2023):15.