Plexin D1 mediates disturbed flow-induced M1 macrophage polarization in atherosclerosis

doi:10.1016/j.heliyon.2023.e17314

CORC > 自动化研究所 > 中国科学院自动化研究所 > 中国科学院分子影像重点实验室

	Plexin D1 mediates disturbed flow-induced M1 macrophage polarization in atherosclerosis
	Zhang, Suhui 2,3; Zhang, Yingqian 3; Zhang, Peng 1; Wei, Zechen 6,7; Ma, Mingrui 2,3; Wang, Wei 3; Tong, Wei 3; Tian, Feng 3; Hui, Hui 6,7; Tian, Jie 4,5,7
刊名	HELIYON
	2023-06-01
卷号	9 期号:6 页码:15
关键词	Plexin D1 Macrophage polarization Disturbed flow Bifurcation lesions Atherosclerosis
DOI	10.1016/j.heliyon.2023.e17314
通讯作者	Hui, Hui(hui.hui@ia.ac.cn) ; Tian, Jie(jie.tian@ia.ac.cn) ; Chen, Yundai(cyundai@vip.163.com)
英文摘要	Atherosclerosis preferentially develops at bifurcations exposed to disturbed flow. Plexin D1 (PLXND1) responds to mechanical forces and drives macrophage accumulation in atherosclerosis. Here, multiple strategies were used to identify the role of PLXND1 in site-specific atherosclerosis. Using computational fluid dynamics and three-dimensional light-sheet fluorescence-microscopy, the elevated PLXND1 in M1 macrophages was mainly distributed in disturbed flow area of ApoE-/ -carotid bifurcation lesions, and visualization of atherosclerosis in vivo was achieved by targeting PLXND1. Subsequently, to simulate the microenvironment of bifurcation lesions in vitro, we co -cultured oxidized low-density lipoprotein (oxLDL)-treated THP-1-derived macrophages with shear-treated human umbilical vein endothelial cells (HUVECs). We found that oscillatory shear induced the increase of PLXND1 in M1 macrophages, and knocking down PLXND1 inhibited M1 polarization. Semaphorin 3E, the ligand of PLXND1 which was highly expressed in plaques, strongly enhanced M1 macrophage polarization via PLXND1 in vitro. Our findings provide in-sights into pathogenesis in site-specific atherosclerosis that PLXND1 mediates disturbed flow -induced M1 macrophage polarization.
资助项目	National Key Research and Development Program of China[2017YFA0700401] ; National Natural Science Foundation of China[81827808] ; National Natural Science Foundation of China[81730050] ; National Natural Science Foundation of China[81870178] ; National Natural Science Foundation of China[81800221] ; National Natural Science Foundation of China[62027901] ; National Natural Science Foundation of China[81527805] ; National Natural Science Foundation of China[81671851] ; CAS Youth Innovation Promotion Association[2018167] ; CAS Key Technology Talent Program ; Project of High -Level Talents Team Introduction in Zhuhai City (Zhuhai)[HLHPTP201703]
WOS关键词	SHEAR-STRESS ; CAROTID BIFURCATION ; PLAQUES ; INFLAMMATION ; ACTIVATION
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	CELL PRESS
WOS记录号	WOS:001042990400001
资助机构	National Key Research and Development Program of China ; National Natural Science Foundation of China ; CAS Youth Innovation Promotion Association ; CAS Key Technology Talent Program ; Project of High -Level Talents Team Introduction in Zhuhai City (Zhuhai)
内容类型	期刊论文
源URL	[http://ir.ia.ac.cn/handle/173211/53957]
专题	自动化研究所_中国科学院分子影像重点实验室
通讯作者	Hui, Hui; Tian, Jie; Chen, Yundai
作者单位	1.Beijing Jiaotong Univ, Sch Comp & Informat Technol, Beijing 100044, Peoples R China 2.Chinese Peoples Liberat Army Gen Hosp, Med Sch Chinese PLA, Beijing 100853, Peoples R China 3.Peoples Liberat Army Gen Hosp, Med Ctr 6, Sr Dept Cardiol, Beijing 100048, Peoples R China 4.Jinan Univ, Zhuhai Peoples Hosp, Zhuhai Precis Med Ctr, Zhuhai 519000, Peoples R China 5.Beihang Univ, Key Lab Big Data Based Precis Med, Minist Ind & Informat Technol China, Beijing 100191, Peoples R China 6.Univ Chinese Acad Sci, Beijing 100080, Peoples R China 7.Chinese Acad Sci, Inst Automat, CAS Key Lab Mol Imaging, Beijing Key Lab Mol Imaging, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Suhui,Zhang, Yingqian,Zhang, Peng,et al. Plexin D1 mediates disturbed flow-induced M1 macrophage polarization in atherosclerosis[J]. HELIYON,2023,9(6):15.
APA	Zhang, Suhui.,Zhang, Yingqian.,Zhang, Peng.,Wei, Zechen.,Ma, Mingrui.,...&Chen, Yundai.(2023).Plexin D1 mediates disturbed flow-induced M1 macrophage polarization in atherosclerosis.HELIYON,9(6),15.
MLA	Zhang, Suhui,et al."Plexin D1 mediates disturbed flow-induced M1 macrophage polarization in atherosclerosis".HELIYON 9.6(2023):15.