[<SUP>68</SUP>Ga]Ga-AUNP-12 PET imaging to assess the PD-L1 status in preclinical and first-in-human study

doi:10.1007/s00259-023-06447-2

CORC > 自动化研究所 > 中国科学院自动化研究所 > 中国科学院分子影像重点实验室

	[⁶⁸Ga]Ga-AUNP-12 PET imaging to assess the PD-L1 status in preclinical and first-in-human study
	Zhou, Ming 2; Xiang, Shijun 2; Zhao, Yajie 2; Tang, Yongxiang 2; Yang, Jinhui 2; Yin, Xiaoqin 2; Tian, Jie 3,4; Hu, Shuo 1,2,5; Du, Yang 3,6
刊名	EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
	2023-09-28
页码	11
关键词	PD-1/PD-L1 Immune checkpoints PET/CT imaging Immunotherapy
ISSN号	1619-7070
DOI	10.1007/s00259-023-06447-2
通讯作者	Tian, Jie(jie.tian@ia.ac.cn) ; Hu, Shuo(hushuo2018@163.com) ; Du, Yang(yang.du@ia.ac.cn)
英文摘要	Purpose PD-L1 PET imaging, as a non-invasive procedure, can perform a real-time, dynamic and quantitative analysis of PD-L1 expression at tumor sites. In this study, we developed a novel peptide-based PET tracer, [(68) Ga]Ga-AUNP-12, for preclinical and first-of-its-kind imaging of PD-L1 expression in patients.Methods Radiosynthesis of [(68) Ga]Ga-AUNP-12 was conducted. Assays for cellular uptake and binding were conducted on the PANC02, CT26, and B16F10 cell lines. Preclinical models were used to investigate its biodistribution, imaging capacity, and pharmacokinetics. Furthermore, interferon-gamma (IFN-gamma) was used for development of an animal model with high PD-L1 expression for targeted PET imaging and efficacy evaluation of PD-L1 blocking therapy. In healthy volunteers and cancer patients, the PD-L1 imaging, radiation dosimetry, safety, and biodistribution were further evaluated.Results In vitro and in vivo animal studies showed that [(68) Ga]Ga-AUNP-12 PET imaging displayed a high specificity in evaluating PD-L1 expression. The radiochemical yield of [(68) Ga]Ga-AUNP-12 was 71.7 +/- 8.2%. Additionally, its molar activity and radiochemical purity were satisfactory. The B16F10 tumor was visualized with the tumor uptake of 6.86 +/- 0.71% ID/g and tumor-to-muscle ratio of 6.83 +/- 0.36 at 60 min after [(68) Ga]Ga-AUNP-12 injection. Furthermore, [(68) Ga]Ga-AUNP-12 PET imaging could sensitively detect the PD-L1 dynamic changes in CT26 tumor xenograft models regulated by IFN-gamma treatment, and correspondingly can effectively guide immunotherapy. Regarding radiation dosimetry, [(68) Ga]Ga-AUNP-12 is safe for human use. The first human study found that [(68) Ga]Ga-AUNP-12 can be rapidly cleared from blood and other nonspecific organs through the kidney excretion, leading to form a clear imaging contrast in the clinical framework. The specificity of [(68) Ga]Ga-AUNP-12 was validated and tumor uptake strongly correlated with the high PD-L1 expression in patients with lung adenocarcinoma and oesophageal squamous cell carcinoma (OSCC).Conclusion [(68) Ga]Ga-AUNP-12 was successfully developed as a PD-L1-specific PET imaging tracer in preclinical and first-in-human studies.
资助项目	National Natural Science Foundation of China[82272111] ; National Natural Science Foundation of China[92159303] ; National Natural Science Foundation of China[62027901] ; National Natural Science Foundation of China[82272045] ; National Natural Science Foundation of China[91859207] ; National Natural Science Foundation of China[81771873] ; National Natural Science Foundation of China[82002887] ; Beijing Natural Science Foundation[7212207] ; Science and Technology Innovation Team Talent Project of Hunan Province[2021RC4056] ; Natural Science Foundation of Hunan Province[2023JJ30976]
WOS关键词	ANTIBODIES ; CANCER ; SAFETY
WOS研究方向	Radiology, Nuclear Medicine & Medical Imaging
语种	英语
出版者	SPRINGER
WOS记录号	WOS:001076672500001
资助机构	National Natural Science Foundation of China ; Beijing Natural Science Foundation ; Science and Technology Innovation Team Talent Project of Hunan Province ; Natural Science Foundation of Hunan Province
内容类型	期刊论文
源URL	[http://ir.ia.ac.cn/handle/173211/53086]
专题	自动化研究所_中国科学院分子影像重点实验室
通讯作者	Tian, Jie; Hu, Shuo; Du, Yang
作者单位	1.Cent South Univ, Xiangya Hosp, Key Lab Biol Nanotechnol Natl Hlth Commiss, Nanotechnol Natl Hlth Commiss, 87 Xiangya Rd, Changsha 410008, Peoples R China 2.Cent South Univ, Xiangya Hosp, Dept Nucl Med, Changsha 410008, Peoples R China 3.Chinese Acad Sci, Inst Automat, CAS Key Lab Mol Imaging, Beijing Key Lab Mol Imaging, 95 Zhongguancun East Rd, Beijing 100190, Peoples R China 4.Beihang Univ, Beijing Adv Innovat Ctr Big Data Based Precis Med, Sch Med, 95 Zhongguancun East Rd, Beijing 100190, Peoples R China 5.Natl Clin Res Ctr Geriatr Disorders, Natl Clin Res Ctr Geriatr Disorders Xiangya, Changsha, Peoples R China 6.Univ Chinese Acad Sci, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Ming,Xiang, Shijun,Zhao, Yajie,et al. [⁶⁸Ga]Ga-AUNP-12 PET imaging to assess the PD-L1 status in preclinical and first-in-human study[J]. EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING,2023:11.
APA	Zhou, Ming.,Xiang, Shijun.,Zhao, Yajie.,Tang, Yongxiang.,Yang, Jinhui.,...&Du, Yang.(2023).[⁶⁸Ga]Ga-AUNP-12 PET imaging to assess the PD-L1 status in preclinical and first-in-human study.EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING,11.
MLA	Zhou, Ming,et al."[⁶⁸Ga]Ga-AUNP-12 PET imaging to assess the PD-L1 status in preclinical and first-in-human study".EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING (2023):11.