NMR-Based Characterization of the Interaction between Yeast Oxa1-CTD and Ribosomes | |
Yong Liu2,3; Jing Yang3; Maosen Ruan3; Huiqin Zhang1,3; Junfeng Wang1,2,3; Yunyan li3 | |
刊名 | International Journal of Molecular Sciences |
2023-09-28 | |
关键词 | Oxa1 insertase C-terminus ribosome interactions NMR structural transitions |
ISSN号 | 1422-0067 |
DOI | doi.org/10.3390/ijms241914657 |
英文摘要 | In mitochondria, the major subunits of oxidative phosphorylation complexes are translated by the mitochondrial ribosome (mito-ribosome). The correct insertion and assembly of these subunits into the inner mitochondrial membrane (IMM) are facilitated by mitochondrial oxidase assembly protein 1 (Oxa1) during the translation process. This co-translational insertion process involves an association between the mito-ribosome and the C-terminus of Oxa1 (Oxa1-CTD) Nuclear magnetic resonance (NMR) methods were mainly used to investigate the structural characterization of yeast Oxa1-CTD and its mode of interaction with the E. coli 70S ribosome. Oxa1-CTD forms a transient-helical structure within the residues P342–Q385, which were reported to form an alpha-helix when combining with the ribosome. Two conserved contact sites that could interact with the ribosome were further identified. The first site was located on the very end of the N-terminus (V321–I327), and the second one encompassed a stretch of amino acid residues I348–Q370. Based on our discoveries and previous reports, a model has been proposed in which Oxa1-CTD interacts with ribosomes, accompanied by transient-to-stable transitions at the second contact site. These observations may enhance our understanding of the potential role of Oxa1-CTD in facilitating the assembly of oxidative phosphorylation complexes and provide insight into the structural characteristics of Oxa1-CTD. |
URL标识 | 查看原文 |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/133241] |
专题 | 合肥物质科学研究院_中科院强磁场科学中心 |
通讯作者 | Yong Liu; Yunyan li |
作者单位 | 1.Institutes of Physical Science and Information Technology, Anhui University, Hefei 230601, China 2.Science Island Branch of Graduate School, University of Science and Technology of China, Hefei 230026, China 3.High Magnetic Field Laboratory, Key Laboratory of High Magnetic Field and Ion Beam Physical Biology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China |
推荐引用方式 GB/T 7714 | Yong Liu,Jing Yang,Maosen Ruan,et al. NMR-Based Characterization of the Interaction between Yeast Oxa1-CTD and Ribosomes[J]. International Journal of Molecular Sciences,2023. |
APA | Yong Liu,Jing Yang,Maosen Ruan,Huiqin Zhang,Junfeng Wang,&Yunyan li.(2023).NMR-Based Characterization of the Interaction between Yeast Oxa1-CTD and Ribosomes.International Journal of Molecular Sciences. |
MLA | Yong Liu,et al."NMR-Based Characterization of the Interaction between Yeast Oxa1-CTD and Ribosomes".International Journal of Molecular Sciences (2023). |
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