Crassaminicella indica sp. nov., a novel thermophilic anaerobic bacterium isolated from a deep- sea hydrothermal vent

doi:10.1099/ijsem.0.005725

CORC > 深海科学与工程研究所 > 中国科学院深海科学与工程研究所 > 深海科学研究部 > 深海生物学研究室 > 深海微生物细胞生物学研究组

	Crassaminicella indica sp. nov., a novel thermophilic anaerobic bacterium isolated from a deep- sea hydrothermal vent
	Jiao, Ze-Xi 8,9; Li, Xue-Gong 5,6,7,9; Zhang, Huan-Huan 4; Xu, Jun 3; Bai, Shi-Jie 2,6; Dai, Jie 1; Lin, Jin 8,9; Zhang, Wei-Jia 5,6,7,9; Qi, Xiao- Qing 6,7,9; Wu, Long- Fei 1,7
刊名	INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
	2023
卷号	73 期号:3 页码:7
关键词	anaerobe Central Indian Ridge Crassaminicella indica thermophile
ISSN号	1466-5026
DOI	10.1099/ijsem.0.005725
通讯作者	Li, Xue-Gong(xuegongli@idsse.ac.cn)
英文摘要	A novel moderately thermophilic heterotrophic bacterium, designated strain 143-21T, was isolated from a deep -sea hydro-thermal chimney sample collected from the Central Indian Ridge at a depth of 2 440 m. Phylogenetic analysis indicated that strain 143-21T belongs to the genus Crassaminicella. It was most closely related to Crassaminicella thermophila SY095T (96.79% 16S rRNA gene sequence similarity) and Crassaminicella profunda Ra1766HT (96.52 %). Genomic analysis showed that strain 143-21T shares 79.79-84.45 % average nucleotide identity and 23.50-29.20 % digital DNA-DNA hybridization with the species of the genus Crassaminicella, respectively. Cells were rod-shaped, non-motile, Gram- positive-staining. Terminal endospores were observed in stationary -phase cells when strain 143-21T was grown on Thermococcales rich medium. Strain 143-21T was able to grow at 30-60 & DEG;C (optimum, 50 & DEG;C), pH 6.5-8.5 (optimum, pH 7.0) and in 1.0-7.0 % NaCl (w/v; optimum 2.0 %, w/v). Strain 143-21T utilized fructose, glucose, maltose, mannose, ribose, N-acetyl -o-(+)-glucosamine and casamino acids, as well as amino acids including glutamate, lysine, histidine and cysteine. The main fermentation products from glucose were acetate (2.07 mM), H2 and CO2. It did not reduce elemental sulphur, sulphate, thiosulphate, sulphite, fumarate, nitrate, nitrite and Fe (III). The pre-dominant cellular fatty acids were C14: 0 (48.8 %), C16: 0 (12.9 %), and summed feature 3 (C16:1 & omega;7c and/or C16:1 & omega;6c; 10.2 %). The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol, as well as two unidenti-fied phospholipids and four unidentified aminolipids. No respiratory quinones were detected. Based on its phylogenetic analysis and physiological characteristics, strain 143-21T is considered to represent a novel species of the genus Crassaminicella, for which the name Crassaminicella indica sp. nov. is proposed. The type strain is strain 143-21T (=DSM 114408T= MCCC 1K06400T).
资助项目	Major Scientific and Technological Projects of Hainan Province[ZDKJ2019011] ; Major Scientific and Technological Projects of Hainan Province[ZDKJ2021028] ; Natural Science Foundation of Hainan Province[321RC616] ; National Natural Science Foundation of China[NSFC 41806174] ; National Natural Science Foundation of China[42 176 121] ; National Natural Science Foundation of China[42 076 127] ; INSB-CNRS[DBM 2021] ; China Scholarship Council (CSC)[202004910195]
WOS关键词	GEN. NOV.
WOS研究方向	Microbiology
语种	英语
出版者	MICROBIOLOGY SOC
WOS记录号	WOS:001044335000028
资助机构	Major Scientific and Technological Projects of Hainan Province ; Natural Science Foundation of Hainan Province ; National Natural Science Foundation of China ; INSB-CNRS ; China Scholarship Council (CSC)
内容类型	期刊论文
源URL	[http://ir.idsse.ac.cn/handle/183446/10439]
专题	深海科学研究部_深海生物学研究室_深海微生物细胞生物学研究组
通讯作者	Li, Xue-Gong
作者单位	1.Aix Marseille Univ, CNRS, LCB, IMM,IM2B, Marseille, France 2.Chinese Acad Sci, Inst Deep sea Sci & Engn, Lab Marine Viruses & Mol Biol, Sanya, Peoples R China 3.Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, State Key Lab Microbial Metab, Shanghai, Peoples R China 4.Shanghai Jiao Tong Univ, Sch Oceanog, Shanghai, Peoples R China 5.Inst Deep sea Life Sci, IDSSE BGI, Hainan Deep sea Technol Lab, Sanya, Hainan, Peoples R China 6.Chinese Acad Sci, Inst Deep sea Sci & Engn, CAS Key Lab Expt Study Deep sea Extreme Condit, Sanya, Peoples R China 7.CNRS Marseille, Int Associated Lab Evolut & Dev Magnetotact Multic, CAS Sanya, Qingdao, Peoples R China 8.Univ Chinese Acad Sci, Beijing, Peoples R China 9.Chinese Acad Sci, Inst Deep sea Sci & Engn, Lab Deep Sea Microbial Cell Biol, Sanya, Peoples R China
推荐引用方式 GB/T 7714	Jiao, Ze-Xi,Li, Xue-Gong,Zhang, Huan-Huan,et al. Crassaminicella indica sp. nov., a novel thermophilic anaerobic bacterium isolated from a deep- sea hydrothermal vent[J]. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY,2023,73(3):7.
APA	Jiao, Ze-Xi.,Li, Xue-Gong.,Zhang, Huan-Huan.,Xu, Jun.,Bai, Shi-Jie.,...&Wu, Long- Fei.(2023).Crassaminicella indica sp. nov., a novel thermophilic anaerobic bacterium isolated from a deep- sea hydrothermal vent.INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY,73(3),7.
MLA	Jiao, Ze-Xi,et al."Crassaminicella indica sp. nov., a novel thermophilic anaerobic bacterium isolated from a deep- sea hydrothermal vent".INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 73.3(2023):7.