Cross-identification of N-Glycans by CE-LIF using two capillary coatings and three labeling dyes

doi:10.1016/j.talanta.2021.123061

CORC > 深海科学与工程研究所 > 中国科学院深海科学与工程研究所 > 深海科学研究部 > 分析测试中心

	Cross-identification of N-Glycans by CE-LIF using two capillary coatings and three labeling dyes
	PingjingLi 1,3; LuluWang 3; RuiGuo 3; HuataoFeng 3; YaJi 3; Si YingLim 3; Bao HuiNg 3; Anna KarenCarrasco Laserna 3; ShaheerKhan 2; Shiaw-MinChen 3
刊名	Talanta
	2021-11-10
卷号	239 页码:10
关键词	Capillary coating Capillary electrophoresis-laser induced fluorescence Fluorogenic dyes Immunoglobulin G N-Glycans
ISSN号	0039-9140
DOI	10.1016/j.talanta.2021.123061
通讯作者	PingjingLi
英文摘要	Recombinant protein biopharmaceuticals comprise a significant portion of the current drug development landscape. The glycosylation profile of these proteins is a key quality parameter as it can affect their safety, efficacy, and stability. However, glycan analysis is challenging because of the complexity of their structures. To overcome this challenge in achieving accurate glycan identification, cross-identification of N-Glycans by CE-LIF method using two capillary coatings and three labeling dyes was developed in this work. This work explored whether complementary separation capabilities can be achieved using homemade polyvinyl alcohol (PVA) coating and commercial Guarant (TM) (Guarant) coating in the analysis of N-glycans. Similar separation profiles were observed using the two capillary coatings, and hence the N-glycan GU databases generated by these coatings were comparable and complementary. The performance of cross-validation by labeling with three fluorescent dyes indicated that low covariance of APTS and Turquoise (TM) labeling can be obtained, and hence these two labeling mechanisms provided better accuracy for the identification of glycans. Superior reproducibility with RSDs less than 1% for all target glycan standards was achieved by the internal standards (IS) method using maltodextrin ladders as additives in the separation buffer. The developed CE-LIF analysis method was applied to the identification of N-glycans in IgG samples.
资助项目	Ministry of Education[R-143-000-B48-114] ; Hundred Talents Program of the Chinese Academy of Sciences, China
WOS关键词	ELECTROPHORESIS ; GLYCOPROTEINS
WOS研究方向	Chemistry
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000759375400007
资助机构	Ministry of Education ; Hundred Talents Program of the Chinese Academy of Sciences, China
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.idsse.ac.cn/handle/183446/8915]
专题	深海科学研究部_分析测试中心
通讯作者	PingjingLi
作者单位	1.Instrumentation Center in Deep-sea Science and Engineering, Science & Technology Tower of Sanya Yazhou Bay, Sanya, 572000, China 2.Thermo Fisher Scientific, 180, Oyster Point Blvd, South San Francisco, CA, 94080, USA 3.Institute of Deep-sea Science and Engineering, Chinese Academy of Sciences, Sanya, Hainan, 572000, China
推荐引用方式 GB/T 7714	PingjingLi,LuluWang,RuiGuo,et al. Cross-identification of N-Glycans by CE-LIF using two capillary coatings and three labeling dyes[J]. Talanta,2021,239:10.
APA	PingjingLi.,LuluWang.,RuiGuo.,HuataoFeng.,YaJi.,...&Sam FongYau Li.(2021).Cross-identification of N-Glycans by CE-LIF using two capillary coatings and three labeling dyes.Talanta,239,10.
MLA	PingjingLi,et al."Cross-identification of N-Glycans by CE-LIF using two capillary coatings and three labeling dyes".Talanta 239(2021):10.