Unravelling the genetic and functional diversity of dominant bacterial communities involved in manure co-composting bioremediation of complex crude oil waste sludge | |
Ubani, Onyedikachi4; Atagana, Harrison I.5; Selvarajan, Ramganesh1,2,4; Ogola, Henry J. O.3,4 | |
刊名 | HELIYON |
2022-02-01 | |
卷号 | 8期号:2页码:15 |
关键词 | Animal manure Bioremediation Bacterial diversity Co-compost Catechol 2,3-dioxygenase |
DOI | 10.1016/j.heliyon.2022.e08945 |
通讯作者 | Ubani, Onyedikachi |
英文摘要 | The present study aimed to characterize the bacterial community and functional diversity in co-composting microcosms of crude oil waste sludge amended with different animal manures, and to evaluate the scope for biostimulation based in situ bioremediation. Gas chromatography-mass spectrometry (GC-MS) analyses revealed enhanced attenuation (>90%) of the total polyaromatic hydrocarbons (PAHs); the manure amendments significantly enhancing (up to 30%) the degradation of high molecular weight (HMW) PAHs. Microbial community analysis showed the dominance (>99% of total sequences) of sequences affiliated to phyla Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes. The core genera enriched were related to hydrocarbon metabolism (Pseudomonas, Delftia, Methylobacterium, Dietzia, Bacillus, Propionibacterium, Bradyrhizobium, Streptomyces, Achromobacter, Microbacterium and Sphingomonas). However, manure-treated samples exhibited high number and heterogeneity of unique operational taxonomic units (OTUs) with enrichment of additional hydrocarbon-degrading bacterial taxa (Proteiniphilum, unclassified Micrococcales, unclassified Lachnospiraceae, Sphingobium and Stenotrophomonas). Thirty-three culturable hydrocarbon-degrading microbes were isolated from the co-composting microcosms and mainly classified into Burkholderia, Sanguibacter, Pseudomonas, Bacillus, Rhodococcus, Lysinibacillus, Microbacterium, Brevibacterium, Geobacillus, Micrococcus, Arthrobacter, Cellulimicrobacterium, Streptomyces Dietzia,etc,. that was additionally affirmed with the presence of catechol 2,3-dioxygenase gene. Finally, enhanced in situ degradation of total (49%), LMW (>75%) and HMW PAHs (>35%) was achieved with an enriched bacterial consortium of these microbes. Overall, these findings suggests that co-composting treatment of crude oil sludge with animal manures selects for intrinsically diverse bacterial community, that could be a driving force behind accelerated bioremediation, and can be exploited for engineered remediation processes. |
资助项目 | South Africa National Research Foundation under the Research and Innovation Support and Advancement[89766] |
WOS关键词 | POLYCYCLIC AROMATIC-HYDROCARBONS ; CONTAMINATED SOILS ; BIODEGRADATION ; BIOAUGMENTATION ; FIELD ; BIOSTIMULATION ; DEGRADATION ; REMEDIATION ; PAHS |
WOS研究方向 | Science & Technology - Other Topics |
语种 | 英语 |
出版者 | ELSEVIER SCI LTD |
WOS记录号 | WOS:000767225100110 |
资助机构 | South Africa National Research Foundation under the Research and Innovation Support and Advancement |
内容类型 | 期刊论文 |
源URL | [http://ir.idsse.ac.cn/handle/183446/9217] |
专题 | 深海科学研究部_地外海洋系统研究室 |
通讯作者 | Ubani, Onyedikachi |
作者单位 | 1.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Lab Extraterr Ocean Syst LEOS, 28 Luhuitou Rd, Sanya 572000, Hainan, Peoples R China 2.JJ Coll Arts & Sci Autonomous, PG Res Dept Microbiol, Pudukkottai 622422, Tamil Nadu, India 3.Jaramogi Oginga Odinga Univ Sci & Technol, Sch Agr & Food Sci, POB 210-40601, Bondo, Kenya 4.Univ South Africa, Coll Agr & Environm Sci, Dept Environm Sci, Florida Campus, ZA-1709 Roodepoort, South Africa 5.Univ South Africa, Coll Sci Engn & Technol, Inst Nanotechnol & Water Sustainabil, Florida Campus, ZA-1709 Roodepoort, South Africa |
推荐引用方式 GB/T 7714 | Ubani, Onyedikachi,Atagana, Harrison I.,Selvarajan, Ramganesh,et al. Unravelling the genetic and functional diversity of dominant bacterial communities involved in manure co-composting bioremediation of complex crude oil waste sludge[J]. HELIYON,2022,8(2):15. |
APA | Ubani, Onyedikachi,Atagana, Harrison I.,Selvarajan, Ramganesh,&Ogola, Henry J. O..(2022).Unravelling the genetic and functional diversity of dominant bacterial communities involved in manure co-composting bioremediation of complex crude oil waste sludge.HELIYON,8(2),15. |
MLA | Ubani, Onyedikachi,et al."Unravelling the genetic and functional diversity of dominant bacterial communities involved in manure co-composting bioremediation of complex crude oil waste sludge".HELIYON 8.2(2022):15. |
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