Phycocyanin - carbon dots nanoprobe for the ratiometric fluorescence determination of peroxynitrite

doi:10.1016/j.saa.2022.121177

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	Phycocyanin - carbon dots nanoprobe for the ratiometric fluorescence determination of peroxynitrite
	J. J. Shao; S. Sun; D. Zhan; L. P. Pan; G. Z. Min; X. B. Li; K. L. Huang; W. Chen; L. K. Yang; X. Y. Liu and N. B. Lin
刊名	Spectrochimica Acta Part a-Molecular and Biomolecular Spectroscopy
	2022
卷号	275 页码:8
ISSN号	1386-1425
DOI	10.1016/j.saa.2022.121177
英文摘要	As a kind of reactive oxygen species, peroxynitrite is related to various diseases closely such as cancer and neurodegenerative diseases. Constructing probes with highly specific ability and a wide linear detection range for peroxynitrite detection is crucial for understanding the pathogenesis of related diseases and optimizing treatments. In this work, we developed a novel luminescent ratiometric fluorescence nanoprobe (PC-CDs) based on carbon dots and phycocyanin. PC-CDs are constructed by amidation reaction between carbon dots and phycocyanin. The nanoprobe we obtained has a good ability of distinguishing peroxynitrite from other reactive oxygen species and interfering substances. Moreover, the linear range of the nanoprobe is 0.5-100 mu M and the limit of detection is 0.5 mu M when detecting peroxynitrite. In the spiked recovery experiments under phosphate buffered saline (PBS) environment, our nanoprobe has a good recovery performance and the recovery is 99% - 104%, which will be beneficial to the further development of peroxynitrite testing and the research progress of related diseases. Finally, we discuss the quenching mechanism of peroxynitrite for nanoprobe, and found that there is the combination of dynamic and static quenching in the quenching process. (C) 2022 Published by Elsevier B.V.
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语种	英语
内容类型	期刊论文
源URL	[http://ir.ciomp.ac.cn/handle/181722/66988]
专题	中国科学院长春光学精密机械与物理研究所
推荐引用方式 GB/T 7714	J. J. Shao,S. Sun,D. Zhan,et al. Phycocyanin - carbon dots nanoprobe for the ratiometric fluorescence determination of peroxynitrite[J]. Spectrochimica Acta Part a-Molecular and Biomolecular Spectroscopy,2022,275:8.
APA	J. J. Shao.,S. Sun.,D. Zhan.,L. P. Pan.,G. Z. Min.,...&X. Y. Liu and N. B. Lin.(2022).Phycocyanin - carbon dots nanoprobe for the ratiometric fluorescence determination of peroxynitrite.Spectrochimica Acta Part a-Molecular and Biomolecular Spectroscopy,275,8.
MLA	J. J. Shao,et al."Phycocyanin - carbon dots nanoprobe for the ratiometric fluorescence determination of peroxynitrite".Spectrochimica Acta Part a-Molecular and Biomolecular Spectroscopy 275(2022):8.