A mitochondria-tracing fluorescent probe for real-time detection of mitochondrial dynamics and hypochlorous acid in live cells

doi:10.1016/j.dyepig.2022.110227

CORC > 长春光学精密机械与物理研究所 > 中国科学院长春光学精密机械与物理研究所

	A mitochondria-tracing fluorescent probe for real-time detection of mitochondrial dynamics and hypochlorous acid in live cells
	N. S. Zhu; X. L. Guo; Y. L. Chang; Z. Shi; L. Y. Jin and S. H. Feng
刊名	Dyes and Pigments
	2022
卷号	201 页码:9
ISSN号	0143-7208
DOI	10.1016/j.dyepig.2022.110227
英文摘要	Mitochondrial dynamics and hypochlorous acid (HOCl) are involved in many physiological and pathological processes. Therefore, the development of real-time analysis tools for biological studies involving mitochondrial dynamics and HOCl is highly significant. Herein, we report a fluorescent probe, RIC, consisting of rhodamine, imidazolium salt, and coumarin, which has high selectivity and sensitivity toward HOCl. RIC has an inherently blue fluorescence signal and can be anchored in mitochondria, which enables it to reveal changes in mitochondrial dynamics. This mitochondria-anchoring characteristic also facilitates the real-time detection of HOCl in mitochondria. To our knowledge, RIC is the first probe to detect mitochondrial dynamics and HOCl in real time. The detection limit of HOCl is as low as 3.8 nM, and RIC analyzes endogenous and exogenous HOCl in live cells. The detection capacity is pH-insensitive in the physiological pH range of mitochondria, and the HOClsensing process is completed within seconds in cuvettes. Furthermore, we prove that the molecular design strategy provides a general synthetic method for constructing new multifunctional probes for HOCl.
URL标识	查看原文
语种	英语
内容类型	期刊论文
源URL	[http://ir.ciomp.ac.cn/handle/181722/66847]
专题	中国科学院长春光学精密机械与物理研究所
推荐引用方式 GB/T 7714	N. S. Zhu,X. L. Guo,Y. L. Chang,et al. A mitochondria-tracing fluorescent probe for real-time detection of mitochondrial dynamics and hypochlorous acid in live cells[J]. Dyes and Pigments,2022,201:9.
APA	N. S. Zhu,X. L. Guo,Y. L. Chang,Z. Shi,&L. Y. Jin and S. H. Feng.(2022).A mitochondria-tracing fluorescent probe for real-time detection of mitochondrial dynamics and hypochlorous acid in live cells.Dyes and Pigments,201,9.
MLA	N. S. Zhu,et al."A mitochondria-tracing fluorescent probe for real-time detection of mitochondrial dynamics and hypochlorous acid in live cells".Dyes and Pigments 201(2022):9.