CHD8 mutations increase gliogenesis to enlarge brain size in the nonhuman primate

	CHD8 mutations increase gliogenesis to enlarge brain size in the nonhuman primate
	Li, Bang 6; Zhao, Hui 5; Tu, Zhuchi 6; Yang, Weili 6; Han, Rui 6; Wang, Lu 6; Luo, Xiaopeng 6; Pan, Mingtian 6; Chen, Xiusheng 6; Zhang, Jiawei 6
刊名	CELL DISCOVERY
	2023
卷号	9 期号:1
ISSN号	2056-5968
英文摘要	Autism spectrum disorder (ASD) is a complex neurodevelopmental condition that affects social interaction and behavior. Mutations in the gene encoding chromodomain helicase DNA-binding protein 8 (CHD8) lead to autism symptoms and macrocephaly by a haploinsufficiency mechanism. However, studies of small animal models showed inconsistent findings about the mechanisms for CHD8 deficiency-mediated autism symptoms and macrocephaly. Using the nonhuman primate as a model system, we found that CRISPR/Cas9-mediated CHD8 mutations in the embryos of cynomolgus monkeys led to increased gliogenesis to cause macrocephaly in cynomolgus monkeys. Disrupting CHD8 in the fetal monkey brain prior to gliogenesis increased the number of glial cells in newborn monkeys. Moreover, knocking down CHD8 via CRISPR/Cas9 in organotypic monkey brain slices from newborn monkeys also enhanced the proliferation of glial cells. Our findings suggest that gliogenesis is critical for brain size in primates and that abnormal gliogenesis may contribute to ASD.
语种	英语
内容类型	期刊论文
源URL	[http://ir.ia.ac.cn/handle/173211/51795]
专题	自动化研究所_脑网络组研究中心
作者单位	1.Yale University 2.Institute of Automation, CAS 3.Guangdong Landau Biotechnol Co Ltd 4.Yuanxi Biotech Inc 5.Institute of Genetics & Developmental Biology, CAS 6.Jinan University
推荐引用方式 GB/T 7714	Li, Bang,Zhao, Hui,Tu, Zhuchi,et al. CHD8 mutations increase gliogenesis to enlarge brain size in the nonhuman primate[J]. CELL DISCOVERY,2023,9(1).
APA	Li, Bang.,Zhao, Hui.,Tu, Zhuchi.,Yang, Weili.,Han, Rui.,...&Li, XiaoJiang.(2023).CHD8 mutations increase gliogenesis to enlarge brain size in the nonhuman primate.CELL DISCOVERY,9(1).
MLA	Li, Bang,et al."CHD8 mutations increase gliogenesis to enlarge brain size in the nonhuman primate".CELL DISCOVERY 9.1(2023).