Characterization and Expression Analysis of Regeneration-Associated Protein (Aj-Orpin) during Intestinal Regeneration in the Sea Cucumber Apostichopus japonicus

doi:10.3390/md20090568

CORC > 海洋研究所 > 中国科学院海洋研究所 > 海洋生态与环境科学重点实验室

	Characterization and Expression Analysis of Regeneration-Associated Protein (Aj-Orpin) during Intestinal Regeneration in the Sea Cucumber Apostichopus japonicus
	Su, Fang 2,3,4,5,6,7; Sun, Lina 2,3,4,5,6,7; Li, Xiaoni 3,7; Cui, Wei 2,3,4,5,6,7; Yang, Hongsheng 1,2,3,4,5,6,7
刊名	MARINE DRUGS
	2022-09-01
卷号	20 期号:9 页码:12
关键词	sea cucumber (Apostichopus japonicus) regeneration holothurians gene function Aj-Orpin
DOI	10.3390/md20090568
通讯作者	Yang, Hongsheng(hshyang@qdio.ac.cn)
英文摘要	Apostichopus japonicus achieves intestinal regeneration in a short period after evisceration, and multiple genes are involved in this process. The transcriptome of A. japonicus was screened for regeneration-associated protein (Aj-Orpin), a gene that is specifically upregulated during intestinal regeneration. The expression and function of Aj-Orpin were identified and investigated in this study. The 5 ' and 3 ' RACE polymerase chain reaction (PCR) was used to clone the full-length cDNA of Aj-Orpin. The open reading frame codes for a 164 amino-acid protein with an EF-hand_7 domain and overlapping signal peptides and transmembrane regions. Moreover, Aj-Orpin mRNA and protein expression during intestinal regeneration was investigated using real-time quantitative PCR and Western blot. The expression pattern of Aj-Orpin in the regenerating intestine was investigated using immunohistochemistry. The results showed that Aj-Orpin is an exocrine protein with two EF-hand-like calcium-binding domains. Expression levels were higher in the regenerating intestine than in the normal intestine, but protein expression changes lagged behind mRNA expression changes. Aj-Orpin was found to play a role in the formation of blastema and lumen. It was primarily expressed in the serosal layer and submucosa, suggesting that it might be involved in proliferation. These observations lay the foundation for understanding the role of Orpin-like in echinoderm intestinal regeneration.
资助项目	National Natural Science Foundation of China[42076093]
WOS研究方向	Pharmacology & Pharmacy
语种	英语
出版者	MDPI
WOS记录号	WOS:000856717900001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/180813]
专题	海洋研究所_海洋生态与环境科学重点实验室
通讯作者	Yang, Hongsheng
作者单位	1.Chinese Acad Sci, Innovat Seed Design, Wuhan 430071, Peoples R China 2.Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, CAS Engn Lab Marine Ranching, Qingdao 266071, Peoples R China 5.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 6.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 7.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Su, Fang,Sun, Lina,Li, Xiaoni,et al. Characterization and Expression Analysis of Regeneration-Associated Protein (Aj-Orpin) during Intestinal Regeneration in the Sea Cucumber Apostichopus japonicus[J]. MARINE DRUGS,2022,20(9):12.
APA	Su, Fang,Sun, Lina,Li, Xiaoni,Cui, Wei,&Yang, Hongsheng.(2022).Characterization and Expression Analysis of Regeneration-Associated Protein (Aj-Orpin) during Intestinal Regeneration in the Sea Cucumber Apostichopus japonicus.MARINE DRUGS,20(9),12.
MLA	Su, Fang,et al."Characterization and Expression Analysis of Regeneration-Associated Protein (Aj-Orpin) during Intestinal Regeneration in the Sea Cucumber Apostichopus japonicus".MARINE DRUGS 20.9(2022):12.