Exploration of the functional properties of red cuticle patch of redclaw crayfish (Cherax quadricarinatus) by histological and transcriptomic analysis

doi:10.1016/j.aquaculture.2022.738624

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Exploration of the functional properties of red cuticle patch of redclaw crayfish (Cherax quadricarinatus) by histological and transcriptomic analysis
	Zhou, Shuhong 2,4; Jiang, Keyong 4; Liu, Mei 1; Wang, Baojie 4; Wang, Lei 1,3,4
刊名	AQUACULTURE
	2022-12-15
卷号	561 页码:9
关键词	Cherax quadricarinatus Chela Red cuticle patch Histology Transcriptome
ISSN号	0044-8486
DOI	10.1016/j.aquaculture.2022.738624
通讯作者	Wang, Baojie(wangbaojie@qdio.ac.cn) ; Wang, Lei(leiwang@qdio.ac.cn)
英文摘要	The redclaw crayfish (Cherax quadricarinatus) is being farmed on an increasing scale in various tropical and subtropical regions. The most distinctive feature of the adult male redclaw crayfish is the red cuticle patch on the lateral cuticle of the chelae. It is an important indicator of the health status, reproduction, and resistance to predators of this species, but little is known about its functional properties. In this study, we used redclaw crayfish (45.35 +/- 16.74 g) as experimental subjects. Histological comparison of the red cuticle patch on male redclaw crayfish chelae with the cuticle on female redclaw crayfish chelae revealed that the red cuticle patch lacked exocuticle. High performance liquid chromatography analysis demonstrated that the concentration of free astaxanthin in the red cuticle patch was much higher than that in the chelae of female redclaw crayfish. Further analysis of transcriptome data from epithelial tissue of the chelae revealed that genes related to chela structure were highly expressed in the red cuticle patch of males. Immunity-related genes were not specifically expressed in the red cuticle patch, and immune-and growth-related genes that require calcium ion for activation were down-regulated in the patch. Our results indicated that regulation of genes related to astaxanthin binding was not a single up-or down-regulation mode. This study demonstrated structural differences in the chela cuticle between male and female redclaw crayfish and revealed the molecular basis for the functional properties of the red cuticle patch.
资助项目	National Key R&D Program of China[2019YFD0900401] ; Yellow River Delta Industry Leading Talent Project
WOS研究方向	Fisheries ; Marine & Freshwater Biology
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000848337500009
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/180831]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Wang, Baojie; Wang, Lei
作者单位	1.Marine Sci Res Inst Shandong Prov, Qingdao Aquat Organisms Qual Evaluat & Utilizat En, Qingdao 266104, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Shuhong,Jiang, Keyong,Liu, Mei,et al. Exploration of the functional properties of red cuticle patch of redclaw crayfish (Cherax quadricarinatus) by histological and transcriptomic analysis[J]. AQUACULTURE,2022,561:9.
APA	Zhou, Shuhong,Jiang, Keyong,Liu, Mei,Wang, Baojie,&Wang, Lei.(2022).Exploration of the functional properties of red cuticle patch of redclaw crayfish (Cherax quadricarinatus) by histological and transcriptomic analysis.AQUACULTURE,561,9.
MLA	Zhou, Shuhong,et al."Exploration of the functional properties of red cuticle patch of redclaw crayfish (Cherax quadricarinatus) by histological and transcriptomic analysis".AQUACULTURE 561(2022):9.