An electrochemical aptasensor with N protein binding aptamer-complementary oligonucleotide as probe for ultra-sensitive detection of COVID-19

doi:10.1016/j.bios.2022.114436

	An electrochemical aptasensor with N protein binding aptamer-complementary oligonucleotide as probe for ultra-sensitive detection of COVID-19
	Yu, Mengdi 7; Zhang, Xiaohui 7; Zhang, Xin 7; Zahra, Qurat Ul Ain 2,3,4; Huang, Zenghui 7; Chen, Ying 7; Song, Chunxia 7; Song, Min 1; Jiang, Hongjuan 1; Luo, Zhaofeng 5,6
刊名	BIOSENSORS & BIOELECTRONICS
	2022-10-01
卷号	213
关键词	Electrochemical aptasensor Rapid COVID-19 diagnosis N protein detection Regenerable aptamer sensor
ISSN号	0956-5663
DOI	10.1016/j.bios.2022.114436
通讯作者	Luo, Zhaofeng() ; Lu, Ying(luy@ahau.edu.cn)
英文摘要	The emergence of the COVID-19 epidemic has affected the lives of hundreds of millions of people globally. There is no doubt that the development of fast and sensitive detection methods is crucial while the worldwide effective vaccination programs are miles away from actualization. In this study, we have reported an electrochemical N protein aptamer sensor with complementary oligonucleotide as probe for the specific detection of COVID-19. The electrochemical aptasensor was prepared by fixing the double-stranded DNA hybrid obtained by the hybridization of N protein aptamer and its Fc-labeled complementary strand on the surface of a gold electrode. After incubation with the target, the aptamer dissociated from the labeled complementary DNA oligonucleotide hybrid to preferentially bind with N protein in the solution. The concentration of N protein was measured by detecting the changes in electrochemical current signals induced by the conformational transformation of the complementary DNA oligonucleotide left on the electrode surface. The sensor had a linear relationship between the logarithm of the N protein concentration from 10 fM to 100 nM (Delta I-p = 0.098 log C-N protein/fM - 0.08433, R-2 = 0.99), and the detection limitation was 1 fM (S/N = 3). The electrochemical aptamer sensor was applied to test the spiked concentrations of throat swabs and blood samples from three volunteers, and the obtained results proved that the sensor has great potentials for the early detection of COVID-19 in patients.
资助项目	NSFC of China[21305002and 21705002] ; Fundamental Research Funds for the Central Universities[YD2070002016] ; Natural Science Foundation of Education Committee of Anhui Province[KJ2021A0176]
WOS关键词	ACUTE RESPIRATORY SYNDROME ; NUCLEOCAPSID PROTEIN ; SYNDROME CORONAVIRUS ; OUTBREAK ; WUHAN ; BIOSENSOR
WOS研究方向	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
语种	英语
出版者	ELSEVIER ADVANCED TECHNOLOGY
WOS记录号	WOS:000833513100006
资助机构	NSFC of China ; Fundamental Research Funds for the Central Universities ; Natural Science Foundation of Education Committee of Anhui Province
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/132147]
专题	中国科学院合肥物质科学研究院
通讯作者	Luo, Zhaofeng; Lu, Ying
作者单位	1.Affiliated Hosp Anhui Agr Univ, Hefei 230036, Peoples R China 2.Univ Sci & Technol China, Core Facil Ctr Life Sci, Dept Mol Biol & Cell Biol, Hefei 230026, Peoples R China 3.Univ Sci & Technol China, Ctr Biomed Engn, Hefei 230026, Peoples R China 4.Univ Sci & Technol China, Hefei Natl Lab for Phys Sci Microscale, Hefei 230026, Peoples R China 5.Univ Sci & Technol China, Canc Hosp, Chinese Acad Sci,Inst Basic Med & Canc IBMC, Aptamer Select Ctr,Univ Chinese Acad Sci, Hangzhou 230027, Peoples R China 6.Univ Sci & Technol China, Sch Life Sci, Hefei 23002, Peoples R China 7.Anhui Agr Univ, Minist Agr & Rural Affairs, Key Lab Agr Sensors, Dept Appl Chem, Hefei 230036, Peoples R China
推荐引用方式 GB/T 7714	Yu, Mengdi,Zhang, Xiaohui,Zhang, Xin,et al. An electrochemical aptasensor with N protein binding aptamer-complementary oligonucleotide as probe for ultra-sensitive detection of COVID-19[J]. BIOSENSORS & BIOELECTRONICS,2022,213.
APA	Yu, Mengdi.,Zhang, Xiaohui.,Zhang, Xin.,Zahra, Qurat Ul Ain.,Huang, Zenghui.,...&Lu, Ying.(2022).An electrochemical aptasensor with N protein binding aptamer-complementary oligonucleotide as probe for ultra-sensitive detection of COVID-19.BIOSENSORS & BIOELECTRONICS,213.
MLA	Yu, Mengdi,et al."An electrochemical aptasensor with N protein binding aptamer-complementary oligonucleotide as probe for ultra-sensitive detection of COVID-19".BIOSENSORS & BIOELECTRONICS 213(2022).