Analysis of volatile organic compounds from deep airway in the lung through intubation sampling

doi:10.1007/s00216-022-04295-x

	Analysis of volatile organic compounds from deep airway in the lung through intubation sampling
	Xu, Wei 1,3; Zhang, Jin 4; Ding, Houwen 4; Ding, Yueting 4; Zou, Xue 1; Yang, Min 4; Zhou, Qiang 4; Liu, Zhou 4; Zheng, Ling 4; Zuo, Heping 4
刊名	ANALYTICAL AND BIOANALYTICAL CHEMISTRY
	2022-08-26
关键词	VOCs Exhaled breath Intubation Deep airway PTR-MS
ISSN号	1618-2642
DOI	10.1007/s00216-022-04295-x
通讯作者	Zou, Xue(xzou@cmpt.ac.cn) ; Yang, Min(yangmin@ahmu.edu.cn) ; Shen, Chengyin(chyshen@aiofm.ac.cn)
英文摘要	Exhaled volatile organic compounds (VOCs) have been widely applied for the study of disease biomarkers. Oral exhalation and nasal exhalation are two of the most common sampling methods. However, VOCs released from food residues and bacteria in the mouth or upper respiratory tract were also sampled and usually mistaken as that produced from body metabolism. In this study, exhalation from deep airway was first directly collected through intubation sampling and analyzed. The exhalation samples of 35 subjects were collected through a catheter, which was inserted into the trachea or bronchus through the mouth and upper respiratory tract. Then, the VOCs in these samples were detected by proton transfer reaction mass spectrometry (PTR-MS). In addition, fast gas chromatography proton transfer reaction mass spectrometry (FGC-PTR-MS) was used to further determine the VOCs with the same mass-to-charge ratios. The results showed that there was methanol, acetonitrile, ethanol, methyl mercaptan, acetone, isoprene, and phenol in the deep airway. Compared with that in oral exhalation, ethanol, methyl mercaptan, and phenol had lower concentrations. In detail, the median concentrations of ethanol, methyl mercaptan, and phenol were 7.3, 0.6, and 23.9 ppbv, while those in the oral exhalation were 80.0, 5.1, and 71.3 ppbv, respectively, which meant the three VOCs mainly originated from the food residues and bacteria in the mouth or upper respiratory tract, rather than body metabolism. The research results in our study can provide references for expiratory VOC research based on oral and nasal exhalation samplings, which are more feasible in clinical practice.
资助项目	National Natural Science Foundation of China[22076190] ; National Natural Science Foundation of China[21876176] ; National Natural Science Foundation of China[62171433] ; National Natural Science Foundation of China[21705152] ; Youth Innovation Promotion Association, CAS, China[2019432] ; Second Affiliated Hospital of Anhui Medical University[LHJJ2020006] ; Center of Medical Physics and Technology of Hefei Institute of Physical Sciences of Chinese Academy of Sciences[LHJJ2020006] ; HFIPS Director's Fund[BJPY2021B08] ; HFIPS Director's Fund[YZJJ2022QN45] ; HFIPS Director's Fund[YZJJZX202009] ; Anhui Provincial Key RD Program[202104d07020003]
WOS关键词	EXHALED BREATH ; MASS-SPECTROMETRY ; CANCER ; BIOMARKERS ; MARKERS ; QUANTIFICATION ; ESOPHAGEAL ; DIAGNOSIS ; ACETONE
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	SPRINGER HEIDELBERG
WOS记录号	WOS:000844909900001
资助机构	National Natural Science Foundation of China ; Youth Innovation Promotion Association, CAS, China ; Second Affiliated Hospital of Anhui Medical University ; Center of Medical Physics and Technology of Hefei Institute of Physical Sciences of Chinese Academy of Sciences ; HFIPS Director's Fund ; Anhui Provincial Key RD Program
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/131850]
专题	中国科学院合肥物质科学研究院
通讯作者	Zou, Xue; Yang, Min; Shen, Chengyin
作者单位	1.Chinese Acad Sci, Inst Hlth & Med Technol, Hefei Inst Phys Sci, Anhui Prov Key Lab Med Phys & Technol, Hefei 230031, Peoples R China 2.Chinese Acad Sci, Hefei Canc Hosp, Hefei 230031, Peoples R China 3.Univ Sci & Technol China, Hefei 230026, Peoples R China 4.Anhui Med Univ, Hosp 2, Hefei 230601, Peoples R China
推荐引用方式 GB/T 7714	Xu, Wei,Zhang, Jin,Ding, Houwen,et al. Analysis of volatile organic compounds from deep airway in the lung through intubation sampling[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2022.
APA	Xu, Wei.,Zhang, Jin.,Ding, Houwen.,Ding, Yueting.,Zou, Xue.,...&Chu, Yannan.(2022).Analysis of volatile organic compounds from deep airway in the lung through intubation sampling.ANALYTICAL AND BIOANALYTICAL CHEMISTRY.
MLA	Xu, Wei,et al."Analysis of volatile organic compounds from deep airway in the lung through intubation sampling".ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2022).