Single-Molecule Resettable DNA Computing via Magnetic Tweezers

doi:10.1021/acs.nanolett.2c00183

	Single-Molecule Resettable DNA Computing via Magnetic Tweezers
	Pei, Yufeng 2,6; Bian, Tianyuan 1,2; Liu, Yonglin 2,3; Liu, Yan 2,5; Xie, Yujie 4; Song, Jie 2,5
刊名	NANO LETTERS
	2022-04-13
卷号	22
关键词	Single-molecule magnetic tweezers Force-controllable DNA logic computing Resettable computing Toehold-mediated DNA strand displacement
ISSN号	1530-6984
DOI	10.1021/acs.nanolett.2c00183
通讯作者	Song, Jie(sjie@sjtu.edu.cn)
英文摘要	DNA-based Boolean logic computing has emerged as a leading technique in biosensing, diagnosis, and therapeutics. Due to the development of the biological and chemical methods, especially the toehold-mediated DNA strand displacement (TMSD) reaction, different logic gates as well as circuits can be performed. However, most of these methods have been conducted at the bulk level, which may lead to missing information and be less controllable. Herein, we engineered single-molecule DNA computing controlled by stretching forces using magnetic tweezers. By tracking the real-time signals of the DNA extension, the output can be determined at a single base-pair resolution. A kinetics-controllable TMSD reaction was realized in the range of a similar to 19-fold change of the reaction rate by different stretching forces. OR, AND, and NOT gates were also achieved. In addition, resettable DNA computing using force stretching cycles has been further exemplified. Overall, such a real-time, label-free, and force-controlled single-molecule DNA computing system provided new insight into molecular computing.
资助项目	National Natural Science Foundation of China[22161132008] ; National Natural Science Foundation of China[81822024] ; Natural Science Foundation of Shanghai, China[19520714100] ; Natural Science Foundation of Shanghai, China[19ZR1475800] ; Project of Shanghai Jiao Tong University[2019QYA03] ; Project of Shanghai Jiao Tong University[YG2017ZD07] ; Institute of Basic Medicine and Cancer (IBMC), Chinese Academy of Sciences[2020QD04]
WOS关键词	LOGIC ; COMPUTATION ; CIRCUITS
WOS研究方向	Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000812715500001
资助机构	National Natural Science Foundation of China ; Natural Science Foundation of Shanghai, China ; Project of Shanghai Jiao Tong University ; Institute of Basic Medicine and Cancer (IBMC), Chinese Academy of Sciences
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/131315]
专题	中国科学院合肥物质科学研究院
通讯作者	Song, Jie
作者单位	1.Tianjin Univ, Acad Med Engn & Translat Med AMT, Tianjin 300072, Peoples R China 2.Univ Chinese Acad Sci, Chinese Acad Sci, Inst Basic Med & Canc IBMC, Canc Hosp, Hangzhou 310022, Zhejiang, Peoples R China 3.Univ Chinese Acad Sci, Hangzhou Inst Adv Study, Sch Mol Med, Hangzhou 310024, Peoples R China 4.Univ Birmingham, Sch Chem, Birmingham B15 2TT, W Midlands, England 5.Shanghai Jiao Tong Univ, Inst Nano Biomed & Engn, Sch Elect Informat & Elect Engn, Dept Instrument Sci & Engn, Shanghai 200240, Peoples R China 6.Chinese Acad Sci, Univ Chinese Acad Sci, Hangzhou Inst Adv Study, Key Lab Syst Hlth Sci Zhejiang Prov, Hangzhou 310024, Peoples R China
推荐引用方式 GB/T 7714	Pei, Yufeng,Bian, Tianyuan,Liu, Yonglin,et al. Single-Molecule Resettable DNA Computing via Magnetic Tweezers[J]. NANO LETTERS,2022,22.
APA	Pei, Yufeng,Bian, Tianyuan,Liu, Yonglin,Liu, Yan,Xie, Yujie,&Song, Jie.(2022).Single-Molecule Resettable DNA Computing via Magnetic Tweezers.NANO LETTERS,22.
MLA	Pei, Yufeng,et al."Single-Molecule Resettable DNA Computing via Magnetic Tweezers".NANO LETTERS 22(2022).