Oligonucleotide Discrimination Enabled by Tannic Acid-Coordinated Film-Coated Solid-State Nanopores | |
Wei, Guanghao2,4; Hu, Rui2,4; Li, Qiuhui2,4; Lu, Wenlong2,4; Liang, Hanyu1; Nan, Hexin1; Lu, Jing2,3,4,5; Li, Juan1; Zhao, Qing2,3,4,5 | |
刊名 | LANGMUIR |
2022-05-24 | |
卷号 | 38 |
ISSN号 | 0743-7463 |
DOI | 10.1021/acs.langmuir.2c00638 |
通讯作者 | Hu, Rui(hooray@pku.edu.cn) ; Li, Juan(juanli@sjtu.edu.cn) ; Zhao, Qing(zhaoqing@pku.edu.cn) |
英文摘要 | Discrimination of nucleotides serves as the basis for DNA sequencing using solid-state nanopores. However, the translocation of DNA is usually too fast to be detected, not to mention nucleotide discrimination. Here, we utilized polyphenolic TA and Fe3+, an attractive metal-organic thin film, and achieved a fast and robust surface coating for silicon nitride nanopores. The hydrophilic coating layer can greatly reduce the low-frequency noise of an original unstable nanopore, and the nanopore size can be finely tuned in situ at the nanoscale by simply adjusting the relative ratio of Fe3+ and TA monomers. Moreover, the hydrogen bonding interaction formed between the hydroxyl groups provided by TA and the phosphate groups of DNAs significantly increases the residence time of a short double-strand (100 bp) DNA. More importantly, we take advantage of the different strengths of hydrogen bonding interactions between the hydroxyl groups provided by TA and the analytes to discriminate between two oligonucleotide samples (oligodeoxycytidine and oligodeoxyadenosine) with similar sizes and lengths, of which the current signal patterns are significantly different using the coated nanopore. The results shed light on expanding the biochemical functionality of surface coatings on solid-state nanopores for future biomedical applications. |
资助项目 | National Key Research and Development Program of China[2019YFA0707003] ; National Key Research and Development Program of China[2019YFE0114100] ; National Key Research and Development Program of China[2020YFA0909000] ; National Natural Science Foundation of China[NSFC 51872007] ; National Natural Science Foundation of China[52103296] ; National Natural Science Foundation of China[91964101] ; National Natural Science Foundation of China[91959102] ; Joint Laboratory of Offshore Wind Power and Intelligent Energy System[HNKJ20-H88] ; Beijing Municipal Natural Science Foundation[7202094] ; Shanghai Rising-Star Program[19QA1405400] |
WOS关键词 | DNA TRANSLOCATION ; MOLECULES ; HYDROGELS ; PROTEINS ; CATECHOL ; PORES |
WOS研究方向 | Chemistry ; Materials Science |
语种 | 英语 |
出版者 | AMER CHEMICAL SOC |
WOS记录号 | WOS:000803035700020 |
资助机构 | National Key Research and Development Program of China ; National Natural Science Foundation of China ; Joint Laboratory of Offshore Wind Power and Intelligent Energy System ; Beijing Municipal Natural Science Foundation ; Shanghai Rising-Star Program |
内容类型 | 期刊论文 |
源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/131033] |
专题 | 中国科学院合肥物质科学研究院 |
通讯作者 | Hu, Rui; Li, Juan; Zhao, Qing |
作者单位 | 1.Univ Chinese Acad Sci, Canc Hosp, Zhejiang Canc Hosp, Inst Basic Med & Canc IBMC,Chinese Acad Sci, Hangzhou 310022, Zhejiang, Peoples R China 2.Peking Univ, Sch Phys, Frontiers Sci Ctr Nanooptoelect, Beijing 100871, Peoples R China 3.Collaborat Innovat Ctr Quantum Matter, Beijing 100084, Peoples R China 4.Peking Univ, Sch Phys, State Key Lab Mesoscop Phys, Beijing 100871, Peoples R China 5.Peking Univ, Yangtze Delta Inst Optoelect, Nantong 226010, Jiangsu, Peoples R China |
推荐引用方式 GB/T 7714 | Wei, Guanghao,Hu, Rui,Li, Qiuhui,et al. Oligonucleotide Discrimination Enabled by Tannic Acid-Coordinated Film-Coated Solid-State Nanopores[J]. LANGMUIR,2022,38. |
APA | Wei, Guanghao.,Hu, Rui.,Li, Qiuhui.,Lu, Wenlong.,Liang, Hanyu.,...&Zhao, Qing.(2022).Oligonucleotide Discrimination Enabled by Tannic Acid-Coordinated Film-Coated Solid-State Nanopores.LANGMUIR,38. |
MLA | Wei, Guanghao,et al."Oligonucleotide Discrimination Enabled by Tannic Acid-Coordinated Film-Coated Solid-State Nanopores".LANGMUIR 38(2022). |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论