MFAP2 aggravates tumor progression through activating FOXM1/beta-catenin-mediated glycolysis in ovarian cancer

doi:10.1002/kjm2.12546

	MFAP2 aggravates tumor progression through activating FOXM1/beta-catenin-mediated glycolysis in ovarian cancer
	Zhao, Ling-Qin 1,2; Sun, Wei 1,2; Zhang, Ping 1,2; Gao, Wen 1,2; Fang, Chen-Yan 1,2; Zheng, Ai-Wen 1,2
刊名	KAOHSIUNG JOURNAL OF MEDICAL SCIENCES
	2022-05-12
关键词	CCK-8 FOXM1 GLUT1 microfibril-associated protein 2 (MFAP2) ovarian cancer
ISSN号	1607-551X
DOI	10.1002/kjm2.12546
通讯作者	Zheng, Ai-Wen(zhengaiwen12345@163.com)
英文摘要	Ovarian cancer is one of the most common gynecological tumors that seriously endanger the health and quality of life of women. Microfibril-associated protein 2 (MFAP2) has been demonstrated to play crucial roles in the development of multiple tumors. However, the function of MFAP2 in ovarian cancer remains unclear. In this study, we found that MFAP2 was upregulated in ovarian cancer and cells and was positively correlated with FOXM1 and glycolysis-related genes. The results of Cell Count Kit-8, colony formation, and flow cytometry assays indicated that MFAP2 promoted cell proliferation. In addition, MFAP2 promotes cell proliferation, glucose uptake, lactate production; increases ATP levels, extracellular acidification ratio, and oxygen consumption ratio in ovarian cancer cells and increases the expression of glycolytic proteins. Further mechanistic analysis suggests that MFAP2 promotes FOXM1/beta-catenin-mediated glycolysis signaling in ovarian cancer cells. Knockdown of MFAP2 inhibits ovarian cancer xenograft tumor growth and expression of Ki-67, MFAP2, FOXM1, GLUT1, HK2, and beta-catenin in mice. In conclusion, MFAP2 promotes cell proliferation and glycolysis by modulating the FOXM1/beta-catenin signaling pathway in ovarian cancer, which may offer a fresh insight into the treatment of ovarian cancer in the glycolysis pathway.
资助项目	Medical Science Project of Zhejiang Province[2022KY688]
WOS关键词	GASTRIC-CANCER ; FOXM1 PROMOTES ; MICROFIBRILS ; METASTASIS ; METABOLISM ; BIOMARKERS ; CELLS
WOS研究方向	Research & Experimental Medicine
语种	英语
出版者	WILEY
WOS记录号	WOS:000793718700001
资助机构	Medical Science Project of Zhejiang Province
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/130821]
专题	中国科学院合肥物质科学研究院
通讯作者	Zheng, Ai-Wen
作者单位	1.Univ Chinese Acad Sci, Canc Hosp, Dept Gynecol Oncol, Zhejiang Canc Hosp, East Banshan Rd, Hangzhou 310022, Peoples R China 2.Chinese Acad Sci, Inst Basic Med & Canc IBMC, Hangzhou, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Ling-Qin,Sun, Wei,Zhang, Ping,et al. MFAP2 aggravates tumor progression through activating FOXM1/beta-catenin-mediated glycolysis in ovarian cancer[J]. KAOHSIUNG JOURNAL OF MEDICAL SCIENCES,2022.
APA	Zhao, Ling-Qin,Sun, Wei,Zhang, Ping,Gao, Wen,Fang, Chen-Yan,&Zheng, Ai-Wen.(2022).MFAP2 aggravates tumor progression through activating FOXM1/beta-catenin-mediated glycolysis in ovarian cancer.KAOHSIUNG JOURNAL OF MEDICAL SCIENCES.
MLA	Zhao, Ling-Qin,et al."MFAP2 aggravates tumor progression through activating FOXM1/beta-catenin-mediated glycolysis in ovarian cancer".KAOHSIUNG JOURNAL OF MEDICAL SCIENCES (2022).