Intelligent and robust DNA robots capable of swarming into leakless nonlinear amplification in response to a trigger

doi:10.1039/d2nh00018k

	Intelligent and robust DNA robots capable of swarming into leakless nonlinear amplification in response to a trigger
	Li, Shaofei 2,3,4; Cheng, Yizhuang 3,4; Qin, Miao 3,4; Zhou, Guoliang 3,4; Li, Pan 4; Yang, Liangbao 1,4
刊名	NANOSCALE HORIZONS
	2022-04-22
ISSN号	2055-6756
DOI	10.1039/d2nh00018k
通讯作者	Yang, Liangbao(lbyang@iim.ac.cn)
英文摘要	Nonlinear DNA signal amplification with an enzyme-free isothermal self-assembly process is uniquely useful in nanotechnology and nanomedicine. However, progress in this direction is hampered by the lack of effective design models of leak-resistant DNA building blocks. Here, we propose two conceptual models of intelligent and robust DNA robots to perform a leakless nonlinear signal amplification in response to a trigger. Two conceptual models are based on super-hairpin nanostructures, which are designed by innovating novel principles in methodology and codifying them into embedded programs. The dynamical and thermodynamical analyses reveal the critical elements and leak-resistant mechanisms of the designed models, and the leak-resistant behaviors of the intelligent DNA robots and morphologies of swarming into nonlinear amplification are separately verified. The applications of the designed models are also illustrated in specific signal amplification and targeted payload enrichment via integration with an aptamer, a fluorescent molecule and surface-enhanced Raman spectroscopy. This work has the potential to serve as design guidelines of intelligent and robust DNA robots and leakless nonlinear DNA amplification, and also as the design blueprint of cargo delivery robots with the performance of swarming into nonlinear amplification in response to a target automatically, facilitating their future applications in biosensing, bioimaging and biomedicine.
资助项目	National Science Foundation of China[21974142] ; Natural Science Research Project of Universities in Anhui Province[K120438026] ; Nature Science Research Project of Anhui Province[1908085QB65]
WOS关键词	HYBRIDIZATION CHAIN-REACTION ; SINGLE ; KINETICS
WOS研究方向	Chemistry ; Science & Technology - Other Topics ; Materials Science
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000792246500001
资助机构	National Science Foundation of China ; Natural Science Research Project of Universities in Anhui Province ; Nature Science Research Project of Anhui Province
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/130779]
专题	中国科学院合肥物质科学研究院
通讯作者	Yang, Liangbao
作者单位	1.Chinese Acad Sci, Hefei Canc Hosp, Hefei 230031, Anhui, Peoples R China 2.Anhui Univ, Sch Life Sci, Hefei 230601, Anhui, Peoples R China 3.Univ Sci & Technol China, Hefei 230026, Anhui, Peoples R China 4.Chinese Acad Sci, Inst Hlth & Med Technol, Hefei Inst Phys Sci, Hefei 230031, Anhui, Peoples R China
推荐引用方式 GB/T 7714	Li, Shaofei,Cheng, Yizhuang,Qin, Miao,et al. Intelligent and robust DNA robots capable of swarming into leakless nonlinear amplification in response to a trigger[J]. NANOSCALE HORIZONS,2022.
APA	Li, Shaofei,Cheng, Yizhuang,Qin, Miao,Zhou, Guoliang,Li, Pan,&Yang, Liangbao.(2022).Intelligent and robust DNA robots capable of swarming into leakless nonlinear amplification in response to a trigger.NANOSCALE HORIZONS.
MLA	Li, Shaofei,et al."Intelligent and robust DNA robots capable of swarming into leakless nonlinear amplification in response to a trigger".NANOSCALE HORIZONS (2022).