The Rostral Ventromedial and Lateral Medulla Are the Major Areas Responsive to Lung Cancer Progression among Brainstem Lung-Innervating Nuclei

doi:10.3390/brainsci12111486

	The Rostral Ventromedial and Lateral Medulla Are the Major Areas Responsive to Lung Cancer Progression among Brainstem Lung-Innervating Nuclei
	Chen, Mo 5; Jiao, Yingfu 4; Shi, Yumiao 4; Xu, Saihong 4; Tang, Dan 4; Chen, Sihan 4; Gao, Po 4; Zhang, Xindi 2; Zhao, Xiaojing 2; Cai, Mengmeng 3
刊名	BRAIN SCIENCES
	2022-11-01
卷号	12
关键词	lung cancer brain-lung axis crosstalk brainstem nuclei vagal core c-Fos pseudorabies virus retrograde tracing neural circuit
DOI	10.3390/brainsci12111486
通讯作者	Yu, Weifeng(ywf808@yeah.net) ; Xie, Kangjie(xiekj9261@126.com)
英文摘要	In recent years, the information crosstalk between the central nervous system and the periphery has been a hot topic, such as the brain-gut axis, brain-lung axis, etc. Among them, some studies have shown that brainstem nuclei activity can significantly affect the progression of peripheral tumor; however, regarding lung cancer, our understanding of the basic characteristics of the lung-innervating brain nuclei responsive to lung cancer progression remains deficient. Therefore, we used the pseudorabies virus for retrograde labeling of nerves to study the neural circuits between the lung and brain. We then established a mouse orthotopic lung cancer model and used the expression of the c-Fos gene in brain regions to characterize activated brain circuits and compared these results with those of the control group. We focused on c-Fos activity in nuclei associated with retrograde tracing regions of the brainstem. We found over 16 nuclei in the whole brain with direct or indirect lung innervation through neural retrograde labeling with the pseudorabies virus. We further revealed that the neuronal activity of the rostral ventrolateral reticular nucleus (RVL), caudal nucleus of Raphe (raphe obscurus nucleus, ROb), Raphe pallidus nucleus (RPa), and ventral gigantocellular reticular nucleus (GiV) in the rostral ventromedial and lateral medulla were significantly changed in an orthotopic lung cancer mouse model by the immunostaining of c-Fos early responsive protein. Thus, the distinctive rostroventral medulla area, functionally closely related to the vagus nerve, likely plays a role in central neural interaction with peripheral lung tumors and deserves future investigation.
资助项目	National Key R&D Program of China[2018YFA0108200] ; Shanghai Committee of Science and Technology[19411951900] ; National Natural Science Foundation of China[32030043] ; National Natural Science Foundation of China[82101287] ; Innovative Research Team of High-level Local Universities in Shanghai[SHSMU-ZDCX20211102] ; Shanghai Municipal Key Clinical Specialty[shslczdzk03601] ; Basic PublicWelfare Research Project in Zhejiang Province[LGD20H010001] ; Shanghai Engineering Research Center of Peri-operative Organ Support and Function Preservation[20DZ2254200] ; Key Specialty Construction Project of Pudong Health and Family Planning Commission of Shanghai[PWZXQ2017-06] ; Innovation Program of Shanghai Municipal Education Commission[2019-01-07-00-01-E00074]
WOS关键词	C1 NEURONS ; PREGANGLIONIC NEURONS ; CNS INNERVATION ; RAPHE OBSCURUS ; PONTINE NUCLEI ; INFLAMMATION ; REFLEX ; NERVE ; STIMULATION ; ACTIVATION
WOS研究方向	Neurosciences & Neurology
语种	英语
出版者	MDPI
WOS记录号	WOS:000883440100001
资助机构	National Key R&D Program of China ; Shanghai Committee of Science and Technology ; National Natural Science Foundation of China ; Innovative Research Team of High-level Local Universities in Shanghai ; Shanghai Municipal Key Clinical Specialty ; Basic PublicWelfare Research Project in Zhejiang Province ; Shanghai Engineering Research Center of Peri-operative Organ Support and Function Preservation ; Key Specialty Construction Project of Pudong Health and Family Planning Commission of Shanghai ; Innovation Program of Shanghai Municipal Education Commission
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/130387]
专题	中国科学院合肥物质科学研究院
通讯作者	Yu, Weifeng; Xie, Kangjie
作者单位	1.Chinese Acad Sci, Univ Chinese Acad Sci, Zhejiang Canc Hosp,Dept Anesthesiol, Canc Hosp,Res Ctr Neuro Oncol Interact,Inst Basic, Hangzhou 310022, Peoples R China 2.Shanghai Jiao Tong Univ, Renji Hosp, Sch Med, Dept Thorac Surg, Shanghai 200127, Peoples R China 3.Nantong Univ, Nantong Peoples Hosp 1, Dept Anesthesiol, Nantong 226001, Peoples R China 4.Shanghai Jiao Tong Univ, Renji Hosp, Sch Med, Dept Anesthesiol, Shanghai 200127, Peoples R China 5.Wannan Med Coll, Grad Sch, Wuhu 241000, Peoples R China
推荐引用方式 GB/T 7714	Chen, Mo,Jiao, Yingfu,Shi, Yumiao,et al. The Rostral Ventromedial and Lateral Medulla Are the Major Areas Responsive to Lung Cancer Progression among Brainstem Lung-Innervating Nuclei[J]. BRAIN SCIENCES,2022,12.
APA	Chen, Mo.,Jiao, Yingfu.,Shi, Yumiao.,Xu, Saihong.,Tang, Dan.,...&Xie, Kangjie.(2022).The Rostral Ventromedial and Lateral Medulla Are the Major Areas Responsive to Lung Cancer Progression among Brainstem Lung-Innervating Nuclei.BRAIN SCIENCES,12.
MLA	Chen, Mo,et al."The Rostral Ventromedial and Lateral Medulla Are the Major Areas Responsive to Lung Cancer Progression among Brainstem Lung-Innervating Nuclei".BRAIN SCIENCES 12(2022).