INVESTIGATION OF BIOMARKERS ASSOCIATED WITH PATHOLOGICAL STAGE OF ESOPHAGEAL ADENOCARCINOMA BY INTEGRATING CO-EXPRESSION NETWORK AND DIFFERENTIAL METHYLATION ANALYSIS

doi:10.19193/0393-6384_2022_4_440

	INVESTIGATION OF BIOMARKERS ASSOCIATED WITH PATHOLOGICAL STAGE OF ESOPHAGEAL ADENOCARCINOMA BY INTEGRATING CO-EXPRESSION NETWORK AND DIFFERENTIAL METHYLATION ANALYSIS
	Chen, Hua-Fei 3; Li, Xiao-Feng 3; Lan, Gang 3; Huang, Li-Chao 3; Zhai, Zhan-Qiang 3; Zhu, You-Cai 3; Wang, Wen-Xian 4; Xu, Chun-Wei 1; He, Xiao 2; Du, Kai-Qi 3
刊名	ACTA MEDICA MEDITERRANEA
	2022
卷号	38
关键词	Esophageal adenocarcinoma co-expression network transcriptomics methylation pathological staging
ISSN号	0393-6384
DOI	10.19193/0393-6384_2022_4_440
通讯作者	He, Xiao(2559472903@qq.com)
英文摘要	Background: Esophageal adenocarcinoma (EAC) is a devastating disease which related to a poor prognosis. Although many efforts have been paid to investigate the progress of EAC, pivotal molecular mechanism involved in the treatment of different tumor stages remains to be demonstrated. Materials and methods: We employ weighted correlation network analysis (WGCNA) and chip analysis methylation pipeline to explore core molecules associated with pathological staging by integrating gene expression and methylation profiles. By constructing a co-expression gene network, we screen out a gene set whose co-expression patterns are related to advanced stages of EAC. Results: Genes affected by abnormal methylation status are recognized by comparing differential methylation sites between cancer tissues and adjacent tissues, which can be distinguished by methylation levels. Based on integrative analysis on epigenetics and transcriptomics, we identified ten co-expressed genes accompanied with differential methylation. Expression levels of 3 genes including EGR2, HOXD8, and RSPO3, are identified to be negatively correlated with methylation values and to be associated with different pathological staging of EAC. These key genes promote tumorigenesis and cell proliferation by altering transcription factors activity and Wnt signaling pathway. Conclusions: Our study provides new insights into discovering characteristic molecules for divergent stages of EAC, which helps to optimize treatment of variable stages.
WOS关键词	BARRETTS-ESOPHAGUS ; DNA METHYLATION ; CANCER ; GENE ; HYPERMETHYLATION ; PROLIFERATION ; PROGRESSION ; VALIDATION ; EXPRESSION ; DYSPLASIA
WOS研究方向	General & Internal Medicine
语种	英语
出版者	CARBONE EDITORE
WOS记录号	WOS:000884059600105
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/130313]
专题	中国科学院合肥物质科学研究院
通讯作者	He, Xiao
作者单位	1.Fujian Med Univ, Fujian Canc Hosp, Dept Pathol, Canc Hosp, Fuzhou 350014, Fujian, Peoples R China 2.Lishui Peoples Hosp, Dept Radiotherapy, Lishui 323000, Zhejiang, Peoples R China 3.Zhejiang Rongjun Hosp, Dept Thorac Dis Ctr, Jiaxing 314000, Zhejiang, Peoples R China 4.Univ Chinese Acad Sci, Chinese Acad Sci, Zhejiang Canc Hosp, Dept Chemotherapy,Canc Hosp,Inst Canc Res & Basic, Hangzhou 310022, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Chen, Hua-Fei,Li, Xiao-Feng,Lan, Gang,et al. INVESTIGATION OF BIOMARKERS ASSOCIATED WITH PATHOLOGICAL STAGE OF ESOPHAGEAL ADENOCARCINOMA BY INTEGRATING CO-EXPRESSION NETWORK AND DIFFERENTIAL METHYLATION ANALYSIS[J]. ACTA MEDICA MEDITERRANEA,2022,38.
APA	Chen, Hua-Fei.,Li, Xiao-Feng.,Lan, Gang.,Huang, Li-Chao.,Zhai, Zhan-Qiang.,...&Du, Kai-Qi.(2022).INVESTIGATION OF BIOMARKERS ASSOCIATED WITH PATHOLOGICAL STAGE OF ESOPHAGEAL ADENOCARCINOMA BY INTEGRATING CO-EXPRESSION NETWORK AND DIFFERENTIAL METHYLATION ANALYSIS.ACTA MEDICA MEDITERRANEA,38.
MLA	Chen, Hua-Fei,et al."INVESTIGATION OF BIOMARKERS ASSOCIATED WITH PATHOLOGICAL STAGE OF ESOPHAGEAL ADENOCARCINOMA BY INTEGRATING CO-EXPRESSION NETWORK AND DIFFERENTIAL METHYLATION ANALYSIS".ACTA MEDICA MEDITERRANEA 38(2022).