Study of the synergistic effect of singlet oxygen with other plasma-generated ROS in fungi inactivation during water disinfection

doi:10.1016/j.scitotenv.2022.156576

	Study of the synergistic effect of singlet oxygen with other plasma-generated ROS in fungi inactivation during water disinfection
	Xu, Hangbo 1; Fang, Cao 1,2; Shao, Changsheng 1,2; Li, Lamei 1,2; Huang, Qing 1
刊名	SCIENCE OF THE TOTAL ENVIRONMENT
	2022-09-10
卷号	838
关键词	Cold atmospheric plasma (CAP) Disinfection Sterilization efficiency and mechanism Synergistic effect Reactive oxygen species (ROS) Singlet oxygen
ISSN号	0048-9697
DOI	10.1016/j.scitotenv.2022.156576
通讯作者	Huang, Qing(huangq@ipp.ac.cn)
英文摘要	Cold atmospheric plasma (CAP) possesses the ability of high-efficiency disinfection. It is reported that mixtures of reactive oxygen species (ROS) including (OH)-O-center dot, O-1(2), O-2(-) and H2O2 generated from CAP have better antimicrobial ability than mimicked solution of mixture of single ROS type, but the reason is not clear. In this study, CAP was applied to treat yeasts in water in order to investigate the fungal inactivation efficiency and mechanism. The results showed that plasma treatment for 5 min could result in >2-log reduction of yeast cells, and application of varied ROS scavengers could significantly increase the yeast survival rate, indicating that (OH)-O-center dot and O-1(2) played the pivotal role in yeast inactivation. Moreover, the synergistic effect of O-1(2) with other plasma-generated ROS was revealed. O-1(2) could diffuse into cells and induce the depolarization of mitochondrial membrane potential (MMP), and different levels of MMP depolarization determined different cell death modes. Mild damage of mitochondria during short-term plasma treatment could lead to apoptosis. For long-term plasma treatment, the cell membrane could be severely damaged by the plasma-generated (OH)-O-center dot, so a large amount of O-1(2) could induce more depolarization of MMP, leading to increase of intracellular O-2(-) and Fe2+ which subsequently caused cell inactivation. O-1(2) could also induce protein aggregation and increase of RIP1/RIP3 necrosome, leading to necroptosis. With participation of O-1(2), endogenous (OH)-O-center dot could also be generated via Fenton and Haber-Weiss reactions during plasma treatment, which potentiated necroptosis. Adding L-His could mitigate membrane damage, inhibit the drop of MMP and the formation of necrosome, and thus prevent the happening of necroptosis. These findings may deepen the understanding of plasma sterilization mechanisms and provide guidance for microbial killing in the environment.
资助项目	National Natural Science Foundation of China[11635013] ; China Postdoctoral Science Foundation[2020M682058] ; Anhui Provincial key research and development plans[202004i07020014] ; Anhui Province Postdoctoral Researcher Scientific Research Funding Project[2021B499]
WOS关键词	MOLECULAR-OXYGEN ; STATE ; MECHANISMS ; DAMAGE
WOS研究方向	Environmental Sciences & Ecology
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000877536400016
资助机构	National Natural Science Foundation of China ; China Postdoctoral Science Foundation ; Anhui Provincial key research and development plans ; Anhui Province Postdoctoral Researcher Scientific Research Funding Project
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/130148]
专题	中国科学院合肥物质科学研究院
通讯作者	Huang, Qing
作者单位	1.Chinese Acad Sci, CAS Key Lab High Magnet Field & Ion Beam Phys Bio, Inst Intelligent Agr, Hefei Inst Phys Sci,Inst Intelligent Machines, Hefei 230031, Peoples R China 2.Univ Sci & Technolagy China, Sci Isl Branch, Grad Sch, Hefei 230026, Peoples R China
推荐引用方式 GB/T 7714	Xu, Hangbo,Fang, Cao,Shao, Changsheng,et al. Study of the synergistic effect of singlet oxygen with other plasma-generated ROS in fungi inactivation during water disinfection[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2022,838.
APA	Xu, Hangbo,Fang, Cao,Shao, Changsheng,Li, Lamei,&Huang, Qing.(2022).Study of the synergistic effect of singlet oxygen with other plasma-generated ROS in fungi inactivation during water disinfection.SCIENCE OF THE TOTAL ENVIRONMENT,838.
MLA	Xu, Hangbo,et al."Study of the synergistic effect of singlet oxygen with other plasma-generated ROS in fungi inactivation during water disinfection".SCIENCE OF THE TOTAL ENVIRONMENT 838(2022).