Whole-Genome Sequencing Reveals Germ Cell Mutagenicity of alpha-Endosulfan in Caenorhabditis elegans

doi:10.1021/acs.est.2c06817

	Whole-Genome Sequencing Reveals Germ Cell Mutagenicity of alpha-Endosulfan in Caenorhabditis elegans
	Cao, Zhenxiao 1,2,3; Wang, Meimei 4; Zhou, Tong 1,3; Xu, An 1,3; Du, Hua 3
刊名	ENVIRONMENTAL SCIENCE & TECHNOLOGY
	2022-10-14
关键词	alpha-endosulfan Caenorhabditis elegans germ cell mutagenicity next-generation sequencing
ISSN号	0013-936X
DOI	10.1021/acs.est.2c06817
通讯作者	Xu, An(anxu@ipp.ac.cn) ; Du, Hua(huadu@ipp.ac.cn)
英文摘要	Endosulfan is an extensively used organochlorine pesticide around the world, which was classified as a persistent organic pollutant (POP) in 2009. Although previous studies have documented the reproductive toxicity of endosulfan in a variety of organisms, little is known about the influence of endosulfan on the genome stability of germ cells and nonexposed progeny. Here we applied whole-genome sequencing to explore the germ cell mutagenicity of alpha-endosulfan in Caenorhabditis elegans (C. elegans). We found that, although low doses of alpha-endosulfan exhibited a minor effect on the reproductive capacity of C. elegans, chronic exposure to 1 mu M alpha-endosulfan significantly increased the mutation frequencies of nonexposed progeny. Further analysis of genome-wide mutation spectra demonstrated that alpha- endosulfan preferentially elicited A:T -> G:C substitutions and clustered mutations. By using worms deficient in DNA damage response genes, our results suggest the involvement of translesion synthesis polymerase ri in modulating alpha-endosulfan-induced mutations in germ cells. Together, these observations reveal the germ cell mutagenicity of alpha-endosulfan in C. elegans and the possible underlying mechanism. In addition, our findings implicate that germ cell mutagenicity might be a necessary consideration for the health risk assessment of environmental chemicals such as POPs.
资助项目	National Natural Science Foundation of China[22006149] ; Hundred Talents Program[E09BR22] ; Anhui Provincial Natural Science Foundation[2008085MB49] ; Anhui Medical University Research Enhancement Program[2021xkjT004]
WOS关键词	ORGANOCHLORINE PESTICIDES ; REPRODUCTIVE TOXICITY ; DNA-DAMAGE ; TRANSLESION ; GENOTOXICITY ; DEGRADATION ; INSECTICIDE ; ISOMERS ; ETA ; AIR
WOS研究方向	Engineering ; Environmental Sciences & Ecology
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000875545100001
资助机构	National Natural Science Foundation of China ; Hundred Talents Program ; Anhui Provincial Natural Science Foundation ; Anhui Medical University Research Enhancement Program
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/129787]
专题	中国科学院合肥物质科学研究院
通讯作者	Xu, An; Du, Hua
作者单位	1.Chinese Acad Sci, Key Lab High Magnet Field & Ion Beam Phys Biol, Hefei 230031, Anhui, Peoples R China 2.Univ Sci & Technol China, Sch Environm Sci & Optoelect Technol, Hefei 230026, Anhui, Peoples R China 3.Chinese Acad Sci, Hefei Inst Phys Sci, Anhui Prov Key Lab Environm Toxicol & Pollut Contr, Hefei 230031, Anhui, Peoples R China 4.Anhui Med Univ, Sch Basic Med Sci, Dept Pathophysiol, Hefei 230032, Anhui, Peoples R China
推荐引用方式 GB/T 7714	Cao, Zhenxiao,Wang, Meimei,Zhou, Tong,et al. Whole-Genome Sequencing Reveals Germ Cell Mutagenicity of alpha-Endosulfan in Caenorhabditis elegans[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2022.
APA	Cao, Zhenxiao,Wang, Meimei,Zhou, Tong,Xu, An,&Du, Hua.(2022).Whole-Genome Sequencing Reveals Germ Cell Mutagenicity of alpha-Endosulfan in Caenorhabditis elegans.ENVIRONMENTAL SCIENCE & TECHNOLOGY.
MLA	Cao, Zhenxiao,et al."Whole-Genome Sequencing Reveals Germ Cell Mutagenicity of alpha-Endosulfan in Caenorhabditis elegans".ENVIRONMENTAL SCIENCE & TECHNOLOGY (2022).