Cuprous ions can disrupt the structure and functions of the RING finger domain of RNF11

doi:10.1039/d2qi00631f

	Cuprous ions can disrupt the structure and functions of the RING finger domain of RNF11
	Wang, Yu 1; Hu, Hongze 1; Yuan, Siming 3; Li, Yunyan 3; Cao, Kaiming 1; Sun, Hongbin 2; Liu, Yangzhong 1
刊名	INORGANIC CHEMISTRY FRONTIERS
	2022-06-06
ISSN号	2052-1553
DOI	10.1039/d2qi00631f
通讯作者	Liu, Yangzhong(liuyz@ustc.edu.cn)
英文摘要	Copper is an essential element that plays crucial roles in a variety of biological processes, while excessive copper is harmful to cells. RNF11 is a RING finger protein associated with cell proliferation and development and metastasis of cancer cells. Here we find that cuprous ions can bind to the RING finger domain of RNF11 with a high binding affinity (disassociation constant 1.80 x 10(-17) M). Cuprous ions are capable of depleting zinc ions from RNF11 and disrupting the structure and function of the protein. Circular dichroism and NMR measurements indicate that Cu(i) binding disrupts the secondary structures of RNF11 and further induces protein aggregation. NMR titration confirms that Cu(i) binds to the Zn(ii) coordination residues. Consequently, cuprous binding interferes with the formation of RNF11/UBE2N complexes, a crucial step of the ubiquitination process. In addition, cellular assays confirmed that copper ions affect its function as a transcriptional regulator for SEC23B, SEC24B, and SEC24D. This result indicates that the alteration of copper homeostasis in cells could influence the RNF11-associated regulation processes.
资助项目	National Key R&D Program of China[2017YFA0505400] ; National Key R&D Program of China[2020YFA0710700] ; National Science Foundation of China[22177109] ; National Science Foundation of China[21877103] ; National Science Foundation of China[52021002]
WOS关键词	PROTEIN RNF11 ; CELL-DEATH ; XIAP
WOS研究方向	Chemistry
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000813557100001
资助机构	National Key R&D Program of China ; National Science Foundation of China
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/129568]
专题	中国科学院合肥物质科学研究院
通讯作者	Liu, Yangzhong
作者单位	1.Univ Sci & Technol China, Dept Chem, Hefei 230026, Anhui, Peoples R China 2.Zhengzhou Univ Light Ind, Sch Food & Biol Engn, Zhengzhou, Peoples R China 3.Chinese Acad Sci, High Magnet Field Lab, Hefei 230031, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yu,Hu, Hongze,Yuan, Siming,et al. Cuprous ions can disrupt the structure and functions of the RING finger domain of RNF11[J]. INORGANIC CHEMISTRY FRONTIERS,2022.
APA	Wang, Yu.,Hu, Hongze.,Yuan, Siming.,Li, Yunyan.,Cao, Kaiming.,...&Liu, Yangzhong.(2022).Cuprous ions can disrupt the structure and functions of the RING finger domain of RNF11.INORGANIC CHEMISTRY FRONTIERS.
MLA	Wang, Yu,et al."Cuprous ions can disrupt the structure and functions of the RING finger domain of RNF11".INORGANIC CHEMISTRY FRONTIERS (2022).