Enhanced resolution of marine viruses with violet side scatter

doi:10.1002/cyto.a.24674

CORC > 海洋研究所 > 中国科学院海洋研究所 > 海洋生态与环境科学重点实验室

	Enhanced resolution of marine viruses with violet side scatter
	Zhao, Yuan 4,5,6; Zhao, Yanchu 3,5,6; Zheng, Shan 2,4,5; Zhao, Li 4,5,6; Zhang, Wuchang 4,5,6; Xiao, Tian 4,5,6; Gregori, Gerald 1
刊名	CYTOMETRY PART A
	2022-08-05
页码	9
关键词	flow cytometry 405 nm side scatter sub-clusters SYBR Green I virus like particles
ISSN号	1552-4922
DOI	10.1002/cyto.a.24674
通讯作者	Gregori, Gerald(gerald.gregori@univ-amu.fr)
英文摘要	Marine viruses make up an essential compartment of the marine ecosystem. They are the most abundant organisms and represent one of the biggest sources of unknown biodiversity. Viruses also have an important impact on bacterial and algal mortality in the ocean, and as such have a major influence on microbial diversity and biogeochemical cycling. However, little is known about the abundance and distribution patterns of viruses across the oceans and seas. Over the last 20 years, flow cytometry has been the technique of choice to detect and count the viral particles in natural samples. Nevertheless, due to their small size, the detection of marine viruses is still extremely challenging. In this article we describe how a new generation of flow cytometer which uses the side scatter (SSC) of violet photons from a 405 nm laser beam helps to improve the resolution for detecting marine viruses. To the best of our knowledge, this is the first report where virioplankton has been detected in aquatic samples using flow cytometry with a 405 nm violet SSC instead of a 488 nm blue SSC.
资助项目	National Natural Science Foundation of China[42076139] ; Open Fund of Pilot National Laboratory for Marine Science and Technology (Qingdao)[QNLM20160RP0311] ; CNRS-NSFC Joint Research Projects Program[CNRS-272110] ; CNRS-NSFC Joint Research Projects Program[NSFC 41711530149] ; 2017-2019 Sino-French Cai Yuanpei Program ; International Research Project-Dynamics and Function of Marine Microorganisms (IRP-DYF2M): insight from physics and remote sensing, CNRS-CAS
WOS研究方向	Biochemistry & Molecular Biology ; Cell Biology
语种	英语
出版者	WILEY
WOS记录号	WOS:000836282700001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/179866]
专题	海洋研究所_海洋生态与环境科学重点实验室
通讯作者	Gregori, Gerald
作者单位	1.Aix Marseille Univ, Toulon Univ, Mediterranean Inst Oceanog UM110, CNRS,IRD, Marseille, France 2.Chinese Acad Sci, Inst Oceanol, Jiaozhou Bay Marine Ecosyst Res Stn, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Peoples R China 5.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 6.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Yuan,Zhao, Yanchu,Zheng, Shan,et al. Enhanced resolution of marine viruses with violet side scatter[J]. CYTOMETRY PART A,2022:9.
APA	Zhao, Yuan.,Zhao, Yanchu.,Zheng, Shan.,Zhao, Li.,Zhang, Wuchang.,...&Gregori, Gerald.(2022).Enhanced resolution of marine viruses with violet side scatter.CYTOMETRY PART A,9.
MLA	Zhao, Yuan,et al."Enhanced resolution of marine viruses with violet side scatter".CYTOMETRY PART A (2022):9.