Bioactivity-guided isolation of cyclooxygenase-2 inhibitors from Saussurea obvallata (DC.) Edgew. Using affinity solid phase extraction assay

	Bioactivity-guided isolation of cyclooxygenase-2 inhibitors from Saussurea obvallata (DC.) Edgew. Using affinity solid phase extraction assay
	Wang, WD; Jiang, L; Zhu, YH; Mei, LJ; Tao, YD; Liu, ZG
刊名	JOURNAL OF ETHNOPHARMACOLOGY
	2022
卷号	284
英文摘要	Ethnopharmacological relevance: Saussurea obvallata (DC.) Edgew. is a traditional Tibetan medicine used for the treatment of inflammation-related diseases, but the scientific validation was very limited. Aim of the study: This study aimed to rapid screen and targeted isolate cyclooxygenase-2 (COX-2) inhibitors from S. obvallata extract. Materials and methods: An efficient ligand-fishing method based on affinity solid phase extraction (A-SPE) combining with HPLC was developed. The identified COX-2 inhibitors were separated using preparative liquid chromatography. In vitro COX-2 inhibition assays were employed to confirm the inhibitory activities of the isolated compounds. In addition, the effect of the isolated compounds on the production of prostaglandin E2 (PGE2) and the expression of COX-2 in LPS-induced RAW 264.7 were evaluated. Results: A total of four phenylpropanoids, isolariciresinol, syringaresinol, pinoresinol and balanophonin were targeted isolated as COX-2 inhibitors with IC50 values of 36.4 +/- 2.6 mu M, 23.1 +/- 1.8 mu M, 3.6 +/- 0.3 mu M and 12.1 +/- 0.9 mu M, respectively. The isolated compounds significantly inhibited LPS-induced NO production in a dose-dependent manner. And, the results of the inhibitory effect on the release of PGE(2) and the expression of COX-2 in LPS-induced macrophages were consistent with A-SPE analysis. Conclusion: The present work demonstrated that the developed A-SPE-HPLC method could successfully targeted isolated COX-2 inhibitors from S. obvallata extract. And, the isolation results indicated that the therapeutic effect of S. obvallata on inflammation-related diseases was partly based on the COX-2 active ingredients.
内容类型	期刊论文
源URL	[http://210.75.249.4/handle/363003/61358]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Wang, WD,Jiang, L,Zhu, YH,et al. Bioactivity-guided isolation of cyclooxygenase-2 inhibitors from Saussurea obvallata (DC.) Edgew. Using affinity solid phase extraction assay[J]. JOURNAL OF ETHNOPHARMACOLOGY,2022,284.
APA	Wang, WD,Jiang, L,Zhu, YH,Mei, LJ,Tao, YD,&Liu, ZG.(2022).Bioactivity-guided isolation of cyclooxygenase-2 inhibitors from Saussurea obvallata (DC.) Edgew. Using affinity solid phase extraction assay.JOURNAL OF ETHNOPHARMACOLOGY,284.
MLA	Wang, WD,et al."Bioactivity-guided isolation of cyclooxygenase-2 inhibitors from Saussurea obvallata (DC.) Edgew. Using affinity solid phase extraction assay".JOURNAL OF ETHNOPHARMACOLOGY 284(2022).