Paternal hypoxia exposure impairs fertilization process and preimplantation embryo development

	Paternal hypoxia exposure impairs fertilization process and preimplantation embryo development
	Tao, HP; Jia, GX; Zhang, XN; Wang, YJ; Li, BY; Yang, QE
刊名	ZYGOTE
	2022
卷号	30 期号:1 页码:48
英文摘要	Environmental hypoxia exposure causes fertility problems in human and animals. Compelling evidence suggests that chronic hypoxia impairs spermatogenesis and reduces sperm motility. However, it is unclear whether paternal hypoxic exposure affects fertilization and early embryo development. In the present study, we exposed male mice to high altitude (3200 m above sea level) for 7 or 60 days to evaluate the effects of hypoxia on sperm quality, zygotic DNA methylation and blastocyst formation. Compared with age-matched controls, hypoxia-treated males exhibited reduced fertility after mating with normoxic females as a result of defects in sperm motility and function. Results of in vitro fertilization (IVF) experiments revealed that 60 days' exposure significantly reduced cleavage and blastocyst rates by 30% and 70%, respectively. Immunohistochemical staining of pronuclear formation indicated that the pronuclear formation process was disturbed and expression of imprinted genes was reduced in early embryos after paternal hypoxia. Overall, the findings of this study suggested that exposing male mice to hypoxia impaired sperm function and affected key events during early embryo development in mammals.
内容类型	期刊论文
源URL	[http://210.75.249.4/handle/363003/61299]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Tao, HP,Jia, GX,Zhang, XN,et al. Paternal hypoxia exposure impairs fertilization process and preimplantation embryo development[J]. ZYGOTE,2022,30(1):48.
APA	Tao, HP,Jia, GX,Zhang, XN,Wang, YJ,Li, BY,&Yang, QE.(2022).Paternal hypoxia exposure impairs fertilization process and preimplantation embryo development.ZYGOTE,30(1),48.
MLA	Tao, HP,et al."Paternal hypoxia exposure impairs fertilization process and preimplantation embryo development".ZYGOTE 30.1(2022):48.