Preparative isolation of maltol glycoside from Dianthus superbus and its anti-inflammatory activity in vitro

	Preparative isolation of maltol glycoside from Dianthus superbus and its anti-inflammatory activity in vitro
	Yuan, C; Dang, J; Han, Y; Liu, C; Yu, S; Lv, Y; Cui, YB; Wang, ZH; Li, G
刊名	RSC ADVANCES
	2022
卷号	12 期号:8 页码:5031
英文摘要	Dianthus superbus is a traditional Chinese medicine that is commonly utilized as a treatment for inflammation, pain, and immunological conditions. In this study, an anti-inflammatory maltol glycoside derived from Dianthus superbus was isolated for the first time via medium and high-pressure liquid chromatography, and at the same time, the in vitro anti-inflammatory activity of this maltol glycoside was preliminarily explored. Initially, crude samples of Dianthus superbus were preprocessed via MCI GEL (R) CHP20P and Spherical C18 medium-pressure chromatography, under the guidance of evaluation of in vitro anti-inflammatory activity. Fr44 was found to be the target fraction, and it was further isolated via two-dimensional reversed-phase/hydrophilic interaction liquid chromatography, yielding > 95% pure and was identified as tunicoside B. MTT assay, nitric oxide and nitric oxide synthase were used to evaluate the effects of tunicoside B on murine macrophage Raw264.7 by nitric oxide synthase assay kit, molecular docking, and western blotting. The results showed that tunicoside B did not affect the viability of cells and exhibited significant anti-inflammatory activity. As far as we know, this is the first report of tunicoside B from Dianthus superbus and the first study on the anti-inflammatory activity of tunicoside B. More importantly, the approach established in this study is expected to provide a theoretical basis for the separation and pharmacological activity study of maltol glycosides from other natural products.
内容类型	期刊论文
源URL	[http://210.75.249.4/handle/363003/61290]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Yuan, C,Dang, J,Han, Y,et al. Preparative isolation of maltol glycoside from Dianthus superbus and its anti-inflammatory activity in vitro[J]. RSC ADVANCES,2022,12(8):5031.
APA	Yuan, C.,Dang, J.,Han, Y.,Liu, C.,Yu, S.,...&Li, G.(2022).Preparative isolation of maltol glycoside from Dianthus superbus and its anti-inflammatory activity in vitro.RSC ADVANCES,12(8),5031.
MLA	Yuan, C,et al."Preparative isolation of maltol glycoside from Dianthus superbus and its anti-inflammatory activity in vitro".RSC ADVANCES 12.8(2022):5031.