Dyestuff chemistry auxiliary instant immune-network label strategy for immunochromatographic detection of chloramphenicol

	Dyestuff chemistry auxiliary instant immune-network label strategy for immunochromatographic detection of chloramphenicol
	Wang, SC; Du, T; Liu, SJ; Li, YC; Wang, Y; Zhang, L; Zhang, DH; Sun, J; Zhu, MQ; Wang, JL
刊名	FOOD CHEMISTRY
	2023
卷号	401
英文摘要	Commercial immunochromatographic assay (ICA) is a convenient tool for controlling antibiotic abuse. Although great efforts have been made to improve the detection performance and quantitative capabilities, simplify the manufacturing process of commercial ICA is rarely mentioned. Here, a proof-of-principle work are developed to solve the above problem. Inspired by dyestuff chemistry, we developed an instant immune-network label strategy by dynamic protonation capacity of neutral red (NR), achieving a desirable labeling efficiency (<1min), and applying for ICA detection of chloramphenicol (CAP). Benefits from the efficiently protonation of NR, lengthy probe production time and organic reagents can be avoided, displaying excellent strip production efficiency and detection performance. Eventually, this strategy presents a visual limit of detection (vLOD) at 3 ng/mL, cut-off value is 9 ng/mL. The assay recoveries in milk and honey were 74.45-107.15 %, with the total RSD of 1.62-6.90 %. We envision that this strategy raises the possibility of commercializing of laboratory prototype products.
内容类型	期刊论文
源URL	[http://210.75.249.4/handle/363003/61036]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Wang, SC,Du, T,Liu, SJ,et al. Dyestuff chemistry auxiliary instant immune-network label strategy for immunochromatographic detection of chloramphenicol[J]. FOOD CHEMISTRY,2023,401.
APA	Wang, SC.,Du, T.,Liu, SJ.,Li, YC.,Wang, Y.,...&Wang, JL.(2023).Dyestuff chemistry auxiliary instant immune-network label strategy for immunochromatographic detection of chloramphenicol.FOOD CHEMISTRY,401.
MLA	Wang, SC,et al."Dyestuff chemistry auxiliary instant immune-network label strategy for immunochromatographic detection of chloramphenicol".FOOD CHEMISTRY 401(2023).