iTRAQ-Based Comparative Proteomic Analysis Provides Insights into Molecular Mechanisms of Salt Tolerance in Sugar Beet (Beta vulgaris L.)

doi:10.3390/ijms19123866

	iTRAQ-Based Comparative Proteomic Analysis Provides Insights into Molecular Mechanisms of Salt Tolerance in Sugar Beet (Beta vulgaris L.)
	Wu, Guo-Qiang 1; Wang, Jin-Long 1; Feng, Rui-Jun 1; Li, Shan-Jia 1; Wang, Chun-Mei 2
刊名	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
	2018-12
卷号	19 期号:12
关键词	sugar beet proteomics salt tolerance relative labeling and absolute quantification of isotope (iTRAQ)
ISSN号	1422-0067
DOI	10.3390/ijms19123866
英文摘要	Salinity is one of the major abiotic stress factors that limit plant growth and crop yield worldwide. To understand the molecular mechanisms and screen the key proteins in response of sugar beet (Beta vulgaris L.) to salt, in the present study, the proteomics of roots and shoots in three-week-old sugar beet plants exposed to 50 mM NaCl for 72 h was investigated by isobaric Tags for Relative and Absolute Quantitation (iTRAQ) technology. The results showed that 105 and 30 differentially expressed proteins (DEPs) were identified in roots and shoots of salt-treated plants compared with untreated plants, respectively. There were 46 proteins up-regulated and 59 proteins down-regulated in roots; and 13 up-regulated proteins and 17 down-regulated proteins found in shoots, respectively. These DEPs were mainly involved in carbohydrate metabolism, energy metabolism, lipid metabolism, biosynthesis of secondary metabolites, transcription, translation, protein folding, sorting, and degradation as well as transport. It is worth emphasizing that some novel salt-responsive proteins were identified, such as PFK5, MDH, KAT2, ACAD10, CYP51, F3H, TAL, SRPR, ZOG, V-H+-ATPase, V-H+-PPase, PIPs, TIPs, and tubulin -2/-1 chain. qRT-PCR analysis showed that six of the selected proteins, including BvPIP1-4, BvVP and BvVAP in root and BvTAL, BvURO-D1, and BvZOG in shoot, displayed good correlation between the expression levels of protein and mRNA. These novel proteins provide a good starting point for further research into their functions using genetic or other approaches. These findings should significantly improve the understanding of the molecular mechanisms involved in salt tolerance of sugar beet plants.
资助项目	Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences[CAAS-ASTIP-2016-LIHPS-08]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	MDPI
WOS记录号	WOS:000455323500168
状态	已发表
内容类型	期刊论文
源URL	[http://119.78.100.223/handle/2XXMBERH/32306]
专题	生命科学与工程学院
通讯作者	Wu, Guo-Qiang
作者单位	1.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730050, Gansu, Peoples R China 2.CAAS, Lanzhou Inst Husb & Pharmaceut Sci, Lanzhou 730050, Gansu, Peoples R China
推荐引用方式 GB/T 7714	Wu, Guo-Qiang,Wang, Jin-Long,Feng, Rui-Jun,et al. iTRAQ-Based Comparative Proteomic Analysis Provides Insights into Molecular Mechanisms of Salt Tolerance in Sugar Beet (Beta vulgaris L.)[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2018,19(12).
APA	Wu, Guo-Qiang,Wang, Jin-Long,Feng, Rui-Jun,Li, Shan-Jia,&Wang, Chun-Mei.(2018).iTRAQ-Based Comparative Proteomic Analysis Provides Insights into Molecular Mechanisms of Salt Tolerance in Sugar Beet (Beta vulgaris L.).INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,19(12).
MLA	Wu, Guo-Qiang,et al."iTRAQ-Based Comparative Proteomic Analysis Provides Insights into Molecular Mechanisms of Salt Tolerance in Sugar Beet (Beta vulgaris L.)".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 19.12(2018).