Toxicity mechanism of patulin on 293 T cells and correlation analysis of Caspase family

doi:10.1093/toxres/tfac053

	Toxicity mechanism of patulin on 293 T cells and correlation analysis of Caspase family
	Zhang, Baigang; Xu, Dongmei; Shao, Lin; Liang, Hairong; Li, Jinliang; Huang, Chenghui
刊名	TOXICOLOGY RESEARCH
	2022-08-01
关键词	patulin 293 T cells oxidative stress apoptosis mitochondrial
ISSN号	2045-452X
DOI	10.1093/toxres/tfac053
英文摘要	Patulin (PAT), a kind of mycotoxin, is a widely disseminated mycotoxin found in agricultural products. Although the existing research results show that PAT can cause nerve, immune, and skin toxicities, resulting in heart, liver, and kidney damages. However, evidence on the underlying mechanisms of PAT is still lacking. Present study aims to investigate the renal toxicity and related mechanisms of PAT on 293 T cells. Cell Counting Kit-8 method was used to reveal the dose-effect relationship and the time-effect relationship of PAT toxicity. Trypan blue staining and Hoechst 33342 staining were used to analyze PAT, which induced apoptosis on 293 T cells. Superoxide-dismutase (SOD), GSH, and malondialdehyde (MDA) were used to measure the changes of oxidative stress status of 293 T cells induced by PAT. The changes of reactive oxygen species (ROS) and ATP in mitochondria indicate the role of mitochondria when PAT induced cell damage and apoptosis. Through Cyt-C release assay analysis, caspase activity change, and correlation analysis, the potential mechanism of mitochondrial apoptosis pathway was proved. Results demonstrated that PAT significantly induced cell injury, and with the increase of time and concentration, the cell survival rate decreased significantly. Hoechst 33342 staining and Trypan blue staining showed that apoptosis rate was elevated by PAT. As PAT concentration increased, intracellular SOD, glutathion peroxidase activities were decreased and the MDA content was increased. The decrease of intracellular ATP level and accumulation of ROS content indicated an increased permeability of the mitochondrial membrane. Overexpression of Cyt-C activated the cascade reaction of caspase enzyme, leading to apoptosis. The results of enzyme activity assay and correlation analysis indicated that caspase 3 was the most critical caspase in the cascade system and that it was most correlated with caspase 8 and caspase 9.
WOS研究方向	Toxicology
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000844233100001
内容类型	期刊论文
源URL	[http://ir.lut.edu.cn/handle/2XXMBERH/159784]
专题	生命科学与工程学院
作者单位	Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730050, Gansu, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Baigang,Xu, Dongmei,Shao, Lin,et al. Toxicity mechanism of patulin on 293 T cells and correlation analysis of Caspase family[J]. TOXICOLOGY RESEARCH,2022.
APA	Zhang, Baigang,Xu, Dongmei,Shao, Lin,Liang, Hairong,Li, Jinliang,&Huang, Chenghui.(2022).Toxicity mechanism of patulin on 293 T cells and correlation analysis of Caspase family.TOXICOLOGY RESEARCH.
MLA	Zhang, Baigang,et al."Toxicity mechanism of patulin on 293 T cells and correlation analysis of Caspase family".TOXICOLOGY RESEARCH (2022).