Improved growth and metabolite accumulation in Codonopsis pilosula (Franch.) Nannf. by inoculation with the endophytic Geobacillu sp. RHBA19 and Pseudomonas fluorescens RHBA17

doi:10.1016/j.jplph.2022.153718

	Improved growth and metabolite accumulation in Codonopsis pilosula (Franch.) Nannf. by inoculation with the endophytic Geobacillu sp. RHBA19 and Pseudomonas fluorescens RHBA17
	Zhu, Ning 2; Meng, Tongtong 1; Li, Shaowei 3; Yu, Chenqun 3; Tang, Dan 2,4; Wang, Yonggang 2,4; Leng, Feifan 2; Ma, Jianzhong 2
刊名	JOURNAL OF PLANT PHYSIOLOGY
	2022-07-01
卷号	274
关键词	Codonopsis pilosula (Franch ) Nannf Endophytic bacteria Interaction Plant physiology Metabolite accumulation
ISSN号	0176-1617
DOI	10.1016/j.jplph.2022.153718
英文摘要	In this study, we focused on the plant-growth-promoting properties of two strains isolated from Codonopsis pilosula, and the effect of inoculation with different strain treatments on physiological and metabolite accumulation of C. pilosula. The strains RHBA19 and RHBA17 were isolated and identified as Geobacillu sp. and Pseudomonas fluorescens, respectively. The two strains produced indole acetic acid (IAA), siderophore, biofilm, and various exoenzymes. Based on the pot experiments, inoculation of RHBA19 (G group) and the two mixed bacteria (M group) significantly improved the growth, root development, and photosynthesis of C. pilosula. Compared with the no-inoculation group (CK), the total polysaccharide content of root in the G and M groups was dramatically enhanced by 59.27% and 96.07%, and the lobetyolin (root) improved by 58.4% and 66.0%, respectively. After inoculation with bacteria agents, the activities of antioxidant enzymes (CAT, POD, SOD) of C. pilosula increased differentially. Inoculation with two types of bacterial agents significantly increased the activities of sucrose synthase (SS) and sucrose phosphate synthase (SPS) in root, and phenylalanine ammonia lyase (PAL) in leaf of C. pilosula. In addition, the content of signaling molecules (NO and H2O2) in three types of tissue increased significantly. The magnitude of these results was higher with mixtures than with individual strains. These results imply that the two types of bacterial agents induce physiological metabolism changes to accumulate polysaccharides and lobetyolin by regulating stress resistance enzymes and signal molecules, especially NO and H2O2.
WOS研究方向	Plant Sciences
语种	英语
出版者	ELSEVIER GMBH
WOS记录号	WOS:000808278800002
内容类型	期刊论文
源URL	[http://ir.lut.edu.cn/handle/2XXMBERH/158924]
专题	生命科学与工程学院
作者单位	1.Lanzhou Univ Technol, Sch Petrochem Engn, Lanzhou 730050, Peoples R China; 2.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730050, Peoples R China; 3.Chinese Acad Sci, Inst Geog Sci & Nat Resources Res, Key Lab Ecosyst Network Observat & Modelling, Lhasa Natl Ecol Res Stn, Beijing 100101, Peoples R China; 4.Lanzhou Univ Technol, Sch Life Sci & Engn, Langongping Rd 287, Lanzhou, Gansu Province, Peoples R China
推荐引用方式 GB/T 7714	Zhu, Ning,Meng, Tongtong,Li, Shaowei,et al. Improved growth and metabolite accumulation in Codonopsis pilosula (Franch.) Nannf. by inoculation with the endophytic Geobacillu sp. RHBA19 and Pseudomonas fluorescens RHBA17[J]. JOURNAL OF PLANT PHYSIOLOGY,2022,274.
APA	Zhu, Ning.,Meng, Tongtong.,Li, Shaowei.,Yu, Chenqun.,Tang, Dan.,...&Ma, Jianzhong.(2022).Improved growth and metabolite accumulation in Codonopsis pilosula (Franch.) Nannf. by inoculation with the endophytic Geobacillu sp. RHBA19 and Pseudomonas fluorescens RHBA17.JOURNAL OF PLANT PHYSIOLOGY,274.
MLA	Zhu, Ning,et al."Improved growth and metabolite accumulation in Codonopsis pilosula (Franch.) Nannf. by inoculation with the endophytic Geobacillu sp. RHBA19 and Pseudomonas fluorescens RHBA17".JOURNAL OF PLANT PHYSIOLOGY 274(2022).