Extraction kinetics, thermodynamics, rheological properties and anti-BVDV activity of the hot water assisted extraction of Glycyrrhiza polysaccharide

doi:10.1039/d0fo00608d

	Extraction kinetics, thermodynamics, rheological properties and anti-BVDV activity of the hot water assisted extraction of Glycyrrhiza polysaccharide
	Wang, Yonggang 1; Wang, Xinjian 1; Zhang, Kang 2; Zhang, Xuan 1; Li, Shaowei 3; Y., Li ; W., Fan ; F., Leng ; M., Yang ; J., Chen
刊名	Food and Function
	2020-05-01
卷号	11 期号:5 页码:4067-4080
关键词	Atomic force microscopy Circular dichroism spectroscopy Dichroism Fourier transform infrared spectroscopy High performance liquid chromatography Mammals Non Newtonian flow Physicochemical properties Rheology Scanning electron microscopy Thermodynamics Viruses Antiviral activities Bovine viral diarrhea virus Extraction temperatures Fourier transform infra red (FTIR) spectroscopy Inhibitory activity Molecular aggregation Optimum concentration Rheological property
ISSN号	20426496
DOI	10.1039/d0fo00608d
英文摘要	The extraction kinetics and thermodynamic parameters of Glycyrrhiza polysaccharide (GP) were studied, and its rheological properties and antiviral activity were evaluated. The results showed that the extraction process could be fitted to Fick's second law of diffusion. The optimum concentration (97.62 mg mL-1) was obtained at a solid-liquid ratio of 1:15, (g mL-1), an extraction time of 120 min and an extraction temperature of 80 °C. The whole extraction process was spontaneous and endothermic. GP was shown to be an acid glycoprotein with a complex structure using high performance liquid chromatography (HPLC), circular dichroism (CD) and Fourier-transform infrared spectroscopy (FT-IR). A study of its rheological properties showed that GP has the characteristics of a typical non-Newtonian pseudoplastic fluid and that its viscosity could be significantly affected by temperature, pH and the presence of other ions. Branched and soft fiber structures with irregular molecular aggregation were observed by scanning electron microscopy (SEM) and atomic force microscopy (AFM). Moreover, GP showed good inhibitory activity against bovine viral diarrhea virus (BVDV) via the regulation of the relative expression levels of the IRF-1 and IRF-3 genes in MDBK cells. This activity was found to be dependent on the physicochemical and structural properties of GP. These findings imply that GP can be considered as a natural source of active material for the prevention of viral disease. © The Royal Society of Chemistry.
WOS研究方向	Biochemistry & Molecular Biology ; Food Science & Technology
语种	英语
出版者	Royal Society of Chemistry
WOS记录号	WOS:000538041500023
内容类型	期刊论文
源URL	[http://ir.lut.edu.cn/handle/2XXMBERH/151110]
专题	生命科学与工程学院
作者单位	1.School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou; 730050, China; 2.Lanzhou Institute of Husbandry and Pharmaceutical Sciences of CAAS, Lanzhou; 730050, China; 3.Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing; 100101, China; 4.Chongqing Key Laboratory of Environmental Materials and Remediation Technologies, Chongqing University of Arts and Sciences, China; 5.School of Petrochemical Engineering, Lanzhou University of Technology, Lanzhou; 730050, China
推荐引用方式 GB/T 7714	Wang, Yonggang,Wang, Xinjian,Zhang, Kang,et al. Extraction kinetics, thermodynamics, rheological properties and anti-BVDV activity of the hot water assisted extraction of Glycyrrhiza polysaccharide[J]. Food and Function,2020,11(5):4067-4080.
APA	Wang, Yonggang.,Wang, Xinjian.,Zhang, Kang.,Zhang, Xuan.,Li, Shaowei.,...&J., Chen.(2020).Extraction kinetics, thermodynamics, rheological properties and anti-BVDV activity of the hot water assisted extraction of Glycyrrhiza polysaccharide.Food and Function,11(5),4067-4080.
MLA	Wang, Yonggang,et al."Extraction kinetics, thermodynamics, rheological properties and anti-BVDV activity of the hot water assisted extraction of Glycyrrhiza polysaccharide".Food and Function 11.5(2020):4067-4080.