CircZNF208 enhances the sensitivity to X-rays instead of carbon-ions through the miR-7-5p /SNCA signal axis in non-small-cell lung cancer cells | |
Bingtao Liu2; Hongbin Li1; Xiongxiong Liu2; Feifei Li3; Weiqiang Chen2; Yanbei Kuang2; Xueshan Zhao4; Linying Li2; Boyi Yu2; Xiaodong Jin5 | |
刊名 | Cell Signal |
2021 | |
卷号 | 84期号:84页码:110012 |
关键词 | Non-small cell lung cancer Radio-sensitivity SNCA circZNF208 miR-7-5p |
ISSN号 | 0898-6568 |
DOI | 10.1016/j.cellsig.2021.110012 |
英文摘要 | Background: Mounting evidence suggests that circular RNAs (circRNAs) are closely related to the regulation of gene expression during tumour development. However, the role of circRNAs in modulating the radiosensitivity of non-small cell lung cancer (NSCLC) cells has not been explored. Methods: Transcriptome sequencing was used to explore the expression profiles of circRNAs in NSCLC. The expression level of circRNAs was changed by inducing instantaneous knockdown or overexpression. Changes in proliferation and radiosensitivity of NSCLC cells were investigated using CCK-8, EDU, and clonal survivals. Results: By analysing the circRNA expression profile of NSCLC cells, we found that circRNA ZNF208 (circZNF208) was significantly upregulated in a radioresistant NSCLC cell line (A549-R11), which was acquired from the parental NSCLC cell line A549. Knockout experiments indicated that circZNF208 enhanced the radiosensitivity of A549 and A549-R11 cells to X-rays. Mechanistically, circZNF208 upregulated SNCA expression by acting as a sponge of miR-7-5p and subsequently promoted the resistance of NSCLC cells to low linear energy transfer (LET) X-rays. However, this effect was not observed in NSCLC cells exposed to high-LET carbon ions. Conclusions: Knockdown of circZNF208 altered the radiosensitivity of patients with NSCLC to X-rays but did not significantly change the sensitivity to carbon ions. Therefore, circZNF208 might serve as a potential biomarker and therapeutic target for NSCLC treatment with radiotherapy of different modalities. |
学科主题 | 基础医学 |
URL标识 | 查看原文 |
WOS研究方向 | Cell Biology |
语种 | 英语 |
出版者 | ELSEVIER SCIENCE INC |
WOS记录号 | WOS:000657674900005 |
内容类型 | 期刊论文 |
源URL | [http://ir.lut.edu.cn/handle/2XXMBERH/148470] |
专题 | 生命科学与工程学院 |
通讯作者 | Xiaodong Jin; Qiang Li |
作者单位 | 1.School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China. 2.Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China; Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences, Lanzhou 730000, China; Key Laboratory of Basic Research on Heavy Ion Radiation Appli 3.Northwest Normal University, Lanzhou, Gansu, China. 4.Lanzhou University, Lanzhou 730000, China. 5.Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China; Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences, Lanzhou 730000, China; Key Laboratory of Basic Research on Heavy Ion Radiation Application in Medicine, Gansu Province, Lanzhou 730000, China; University of Chinese Academy of Sciences, Beijing 100049, China. 6.Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China; Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences, Lanzhou 730000, China; Key Laboratory of Basic Research on Heavy Ion Radiation Application in Medicine, Gansu Province, Lanzhou 730000, China; University of Chinese Academy of Sciences, Beijing 100049, China. |
推荐引用方式 GB/T 7714 | Bingtao Liu,Hongbin Li,Xiongxiong Liu,et al. CircZNF208 enhances the sensitivity to X-rays instead of carbon-ions through the miR-7-5p /SNCA signal axis in non-small-cell lung cancer cells[J]. Cell Signal,2021,84(84):110012. |
APA | Bingtao Liu.,Hongbin Li.,Xiongxiong Liu.,Feifei Li.,Weiqiang Chen.,...&Qiang Li.(2021).CircZNF208 enhances the sensitivity to X-rays instead of carbon-ions through the miR-7-5p /SNCA signal axis in non-small-cell lung cancer cells.Cell Signal,84(84),110012. |
MLA | Bingtao Liu,et al."CircZNF208 enhances the sensitivity to X-rays instead of carbon-ions through the miR-7-5p /SNCA signal axis in non-small-cell lung cancer cells".Cell Signal 84.84(2021):110012. |
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