Sequence analysis of potato α-amylase gene amyA2

doi:10.4028/www.scientific.net/AMR.643.56

	Sequence analysis of potato α-amylase gene amyA2
	Wang, Yonggang; Ma, Jianzhong; Ma, Xueqing; Liu, Jinge; Yang, Mingjun
	2013
会议日期	December 22, 2012 - December 23, 2012
会议地点	Yichang, China
关键词	Amino acids Bioinformatics Chemical properties Genes Nanotechnology Active site Amino acid residues Amylase genes Beta-sheet Bioinformatic analysis Catalytic domains Codon usage bias GenBank Open reading frame Physical and chemical properties Potato Sequence analysis Subcellular localizations
卷号	643
DOI	10.4028/www.scientific.net/AMR.643.56
页码	56-59
英文摘要	The sequences of Potato α-Amylase Gene amyA2 was analysised by Bioinformatics, including its codon usage bias, physical and chemical properties, subcellular localization, and conserved structures. The results showed that the cDNA had a 1218 bp open reading frame and was referred to as amyA2, which encodes for an α-amylase with 405 amino acid residues (GenBank accession number: GU134783), and shared 98% identity with a published potato α-amylase (GenBank accession number: M79328.1) at the amino acid level. The amino sequences contains a catalytic domain (PF00128, SM00624) between 20 to 348 and a C-terminal beta-sheet domain between 349-407, which are similar to ones of the amylase family 13. Eight-stranded alpha/beta barrel was also found in the enzyme, which was thought as an active site of α-amylase. © (2013) Trans Tech Publications, Switzerland.
会议录	Advanced Materials Research
会议录出版者	Trans Tech Publications, P.O. Box 1254, Clausthal-Zellerfeld, D-38670, Germany
语种	英语
ISSN号	10226680
内容类型	会议论文
源URL	[http://ir.lut.edu.cn/handle/2XXMBERH/117581]
专题	生命科学与工程学院
作者单位	Lanzhou University of Technology, Lanzhou, China
推荐引用方式 GB/T 7714	Wang, Yonggang,Ma, Jianzhong,Ma, Xueqing,et al. Sequence analysis of potato α-amylase gene amyA2[C]. 见:. Yichang, China. December 22, 2012 - December 23, 2012.