Development of a lateral flow test strip for simultaneous detection of BT-Cry1Ab, BT-Cry1Ac and CP4 EPSPS proteins in genetically modified crops

doi:10.1016/j.foodchem.2020.127627

	Development of a lateral flow test strip for simultaneous detection of BT-Cry1Ab, BT-Cry1Ac and CP4 EPSPS proteins in genetically modified crops
	Zeng, Haijuan 1; Wang, Jinbin 1; Jia, Junwei 1; Wu, Guogan 1; Yang, Qianwen 1,2; Liu, Xiaofeng 2; Tang, Xueming 1
刊名	Food Chemistry
	2021-01-15
卷号	335
关键词	Crops Gold nanoparticles Proteins Sugar beets Genetically modified crops Lateral flow test Multi-component analysis Multiple components Parallel analysis Simultaneous detection Target proteins Visual detection
ISSN号	03088146
DOI	10.1016/j.foodchem.2020.127627
英文摘要	A colloidal gold immunochromatographic strip (ICS) for simultaneous detection of multiple transgenic proteins, including CP4 EPSPS, BT-Cry1Ab and BT-Cry1Ac, was developed in this study. The sensitivity of the strip to the target protein was 5 ng/mL for CP4 EPSPS, 100 ng/mL for BT-Cry1Ab and Cry1Ac, respectively. Parallel analysis for maize, soybean, sugar beet and cotton showed the strip could detect 1% of transgenic content in crops containing BT-Cry1Ab and Cry1Ac, and, at least, 0.1% of content in crops containing CP4 EPSPS. The detection results for seed samples indicated the multicomponent analysis ICS had good accuracy. The analysis could be completed within 10 min and had the advantages of being high-throughput, easy to operate and visual detection. This is the first report of semi-quantitative ICS for detecting three transgenic proteins simultaneously. The developed approach may provide insights into the development of ICS for analyzing simultaneously multiple components in genetically modified crops. © 2020 Elsevier Ltd
语种	英语
出版者	Elsevier Ltd
内容类型	期刊论文
源URL	[http://ir.lut.edu.cn/handle/2XXMBERH/115224]
专题	生命科学与工程学院
作者单位	1.The Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai; 201106, China; 2.School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou; 730050, China
推荐引用方式 GB/T 7714	Zeng, Haijuan,Wang, Jinbin,Jia, Junwei,et al. Development of a lateral flow test strip for simultaneous detection of BT-Cry1Ab, BT-Cry1Ac and CP4 EPSPS proteins in genetically modified crops[J]. Food Chemistry,2021,335.
APA	Zeng, Haijuan.,Wang, Jinbin.,Jia, Junwei.,Wu, Guogan.,Yang, Qianwen.,...&Tang, Xueming.(2021).Development of a lateral flow test strip for simultaneous detection of BT-Cry1Ab, BT-Cry1Ac and CP4 EPSPS proteins in genetically modified crops.Food Chemistry,335.
MLA	Zeng, Haijuan,et al."Development of a lateral flow test strip for simultaneous detection of BT-Cry1Ab, BT-Cry1Ac and CP4 EPSPS proteins in genetically modified crops".Food Chemistry 335(2021).