Isolation and characterization of pepsin-soluble collagen from the skin of peru squid (Dosidicus gigas)

doi:10.1080/10498850.2011.646002

	Isolation and characterization of pepsin-soluble collagen from the skin of peru squid (Dosidicus gigas)
	Fu, Wandong 2; Wang, Yan 1; Zheng, Bin 2; Liao, Miaofei 2; Zhang, Weijie 3
刊名	Journal of Aquatic Food Product Technology
	2013-05-04
卷号	22 期号:3 页码:270-280
关键词	Amino acids Electrophoresis Fourier transform infrared spectroscopy Mammals Molluscs Physicochemical properties Shellfish Sodium chloride Sodium dodecyl sulfate Sulfur compounds Alkaline conditions Amino acid compositions Denaturation temperatures isolation Isolation and characterization NaCl concentration Research and application Sodium dodecyl sulfate-polyacrylamide gel electrophoresis
ISSN号	10498850
DOI	10.1080/10498850.2011.646002
英文摘要	Pepsin-soluble collagen (PSC) was isolated from Peru squid (Dosidicus gigas) skin and physicochemical properties of the PSC were determined. The PSC exhibited a maximum absorbance at 220 nm. Sodium dodecyl sulfate polyacrylamide gel electrophoresis suggested the collagen containing 1 and 2 chain was classified as type I collagen. Amino acid composition indicated that the collagen had lower amino acid content than that of mammalian collagen. Denaturation temperature (Td) of the PSC was 26.8°C. The PSC had relatively high solubility in alkaline condition or NaCl concentrations below 2%. Fourier transform infrared spectroscopy investigations showed the existence of helical arrangements of collagen. The lyophilized collagen had a uniform and regular network structure. These results suggested that Peru squid skin was a potential source of collagen for further research and application. © 2013 Copyright Taylor and Francis Group, LLC.
WOS研究方向	Food Science & Technology
语种	英语
出版者	Taylor and Francis Inc., 325 Chestnut St, Suite 800, Philadelphia, PA 19106, United States
WOS记录号	WOS:000319039100006
内容类型	期刊论文
源URL	[http://ir.lut.edu.cn/handle/2XXMBERH/113766]
专题	生命科学与工程学院
作者单位	1.Zhejiang Ocean University, Zhoushan, China; 2.Zhejiang Marine Development Research Institute, 2119 Donghai West Road, Putuo District, Zhoushan 316100, China; 3.Lanzhou University of Technology, Lanzhou, China
推荐引用方式 GB/T 7714	Fu, Wandong,Wang, Yan,Zheng, Bin,et al. Isolation and characterization of pepsin-soluble collagen from the skin of peru squid (Dosidicus gigas)[J]. Journal of Aquatic Food Product Technology,2013,22(3):270-280.
APA	Fu, Wandong,Wang, Yan,Zheng, Bin,Liao, Miaofei,&Zhang, Weijie.(2013).Isolation and characterization of pepsin-soluble collagen from the skin of peru squid (Dosidicus gigas).Journal of Aquatic Food Product Technology,22(3),270-280.
MLA	Fu, Wandong,et al."Isolation and characterization of pepsin-soluble collagen from the skin of peru squid (Dosidicus gigas)".Journal of Aquatic Food Product Technology 22.3(2013):270-280.