Fluorescence investigation of affinity interaction between bovine serum albumin and triazine dye in reversed micelles

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	Fluorescence investigation of affinity interaction between bovine serum albumin and triazine dye in reversed micelles
	Zhang, TX; Liu, HZ; Chen, JY
刊名	APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
	2001-09-01
卷号	95 期号:3 页码:163-174
关键词	affinity interaction reversed micelles bovine serum albumin Cibacron Blue 3GA fluorescence emission spectra
ISSN号	0273-2289
其他题名	Appl. Biochem. Biotechnol.
中文摘要	The effect of Cibacron Blue 3GA (CB) on the fluorescence emission spectra of bovine serum albumin (BSA) was investigated in cationic reversed micelles formed with cetyltrimethylammonium bromide (CTAB) compared with that in aqueous phase. The anionic CB had electrostatic interactions with cationic CTAB and affinity interactions with BSA in the reversed micelles. The addition of CB in the reversed micellar phase led to a great decrease in the fluorescence intensity of BSA and a remarkable red shift of the wavelength of emission maximum (lambda (max)). The fluorescence intensity of BSA decreased and the lambda (max) decreased 5 nm (blue shift) without the addition of CB in the reversed micellar phase. The fluorescence intensity of BSA with the addition of CB had the strongest value in the aqueous phase with the presence of CTAB, a less strong value in the reversed micellar phase, and a weak value in the aqueous phase without the presence of CTAB. The increase in X max of BSA with the addition of CB in the reversed micellar phase might indicate the decrease in the hydrophobic microenvironment of the Trp residue of BSA, contrary to those microenvironments in the absence of CB.
英文摘要	The effect of Cibacron Blue 3GA (CB) on the fluorescence emission spectra of bovine serum albumin (BSA) was investigated in cationic reversed micelles formed with cetyltrimethylammonium bromide (CTAB) compared with that in aqueous phase. The anionic CB had electrostatic interactions with cationic CTAB and affinity interactions with BSA in the reversed micelles. The addition of CB in the reversed micellar phase led to a great decrease in the fluorescence intensity of BSA and a remarkable red shift of the wavelength of emission maximum (lambda (max)). The fluorescence intensity of BSA decreased and the lambda (max) decreased 5 nm (blue shift) without the addition of CB in the reversed micellar phase. The fluorescence intensity of BSA with the addition of CB had the strongest value in the aqueous phase with the presence of CTAB, a less strong value in the reversed micellar phase, and a weak value in the aqueous phase without the presence of CTAB. The increase in X max of BSA with the addition of CB in the reversed micellar phase might indicate the decrease in the hydrophobic microenvironment of the Trp residue of BSA, contrary to those microenvironments in the absence of CB.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
研究领域[WOS]	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
关键词[WOS]	MYELIN BASIC-PROTEIN ; NONIONIC SURFACTANT ; CIBACRON BLUE ; EXTRACTION ; BIOAFFINITY ; SEPARATION ; TRYPSIN ; DYNAMICS ; SYSTEMS ; ENZYMES
收录类别	SCI
原文出处	://WOS:000171816400002
语种	英语
WOS记录号	WOS:000171816400002
公开日期	2013-11-14
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/5744]
专题	过程工程研究所_研究所（批量导入）
作者单位	Chinese Acad Sci, Inst Chem Met, Lab Separat Sci & Engn, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Zhang, TX,Liu, HZ,Chen, JY. Fluorescence investigation of affinity interaction between bovine serum albumin and triazine dye in reversed micelles[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2001,95(3):163-174.
APA	Zhang, TX,Liu, HZ,&Chen, JY.(2001).Fluorescence investigation of affinity interaction between bovine serum albumin and triazine dye in reversed micelles.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,95(3),163-174.
MLA	Zhang, TX,et al."Fluorescence investigation of affinity interaction between bovine serum albumin and triazine dye in reversed micelles".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 95.3(2001):163-174.