Viable but nonculturable bacteria and their resuscitation: implications for cultivating uncultured marine microorganisms

doi:10.1007/s42995-020-00041-3

CORC > 兰州理工大学 > 兰州理工大学 > 石油化工学院

	Viable but nonculturable bacteria and their resuscitation: implications for cultivating uncultured marine microorganisms
	Zhang, Xiao-Hua 1,2,3,5; Ahmad, Waqar 1,2; Zhu, Xiao-Yu 1,2; Chen, Jixiang 4; Austin, Brian 6
刊名	MARINE LIFE SCIENCE & TECHNOLOGY
	2021-05
卷号	3 期号:2 页码:189-203
关键词	VBNC Cultivating the uncultured Resuscitation Marine microorganisms
ISSN号	2096-6490
DOI	10.1007/s42995-020-00041-3
英文摘要	Culturing has been the cornerstone of microbiology since Robert Koch first successfully cultured bacteria in the late nineteenth century. However, even today, the majority of microorganisms in the marine environment remain uncultivated. There are various explanations for the inability to culture bacteria in the laboratory, including lack of essential nutrients, osmotic support or incubation conditions, low growth rate, development of micro-colonies, and the presence of senescent or viable but nonculturable (VBNC) cells. In the marine environment, many bacteria have been associated with dormancy, as typified by the VBNC state. VBNC refers to a state where bacteria are metabolically active, but are no longer culturable on routine growth media. It is apparently a unique survival strategy that has been adopted by many microorganisms in response to harsh environmental conditions and the bacterial cells in the VBNC state may regain culturability under favorable conditions. The resuscitation of VBNC cells may well be an important way to cultivate the otherwise uncultured microorganisms in marine environments. Many resuscitation stimuli that promote the restoration of culturability have so far been identified; these include sodium pyruvate, quorum sensing autoinducers, resuscitation-promoting factors Rpfs and YeaZ, and catalase. In this review, we focus on the issues associated with bacterial culturability, the diversity of bacteria entering the VBNC state, mechanisms of induction into the VBNC state, resuscitation factors of VBNC cells and implications of VBNC resuscitation stimuli for cultivating these otherwise uncultured microorganisms. Bringing important microorganisms into culture is still important in the era of high-throughput sequencing as their ecological functions in the marine environment can often only be known through isolation and cultivation.
WOS研究方向	Marine & Freshwater Biology
语种	英语
出版者	SPRINGERNATURE
WOS记录号	WOS:000649455400007
内容类型	期刊论文
源URL	[http://ir.lut.edu.cn/handle/2XXMBERH/148497]
专题	石油化工学院
作者单位	1.Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China; 2.Ocean Univ China, Inst Evolut & Marine Biodivers, Qingdao 266003, Peoples R China; 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China; 4.Lanzhou Univ Technol, Sch Petrochem Engn, Lanzhou 730050, Peoples R China; 5.Ocean Univ China, Frontiers Sci Ctr Deep Ocean Multispheres & Earth, Qingdao 266100, Peoples R China; 6.Univ Stirling, Inst Aquaculture, Stirling FK9 4LA, Scotland
推荐引用方式 GB/T 7714	Zhang, Xiao-Hua,Ahmad, Waqar,Zhu, Xiao-Yu,et al. Viable but nonculturable bacteria and their resuscitation: implications for cultivating uncultured marine microorganisms[J]. MARINE LIFE SCIENCE & TECHNOLOGY,2021,3(2):189-203.
APA	Zhang, Xiao-Hua,Ahmad, Waqar,Zhu, Xiao-Yu,Chen, Jixiang,&Austin, Brian.(2021).Viable but nonculturable bacteria and their resuscitation: implications for cultivating uncultured marine microorganisms.MARINE LIFE SCIENCE & TECHNOLOGY,3(2),189-203.
MLA	Zhang, Xiao-Hua,et al."Viable but nonculturable bacteria and their resuscitation: implications for cultivating uncultured marine microorganisms".MARINE LIFE SCIENCE & TECHNOLOGY 3.2(2021):189-203.