The development of RPA and CRISPR-Cas12a based immunoassay strip for sensitive detection of genetically modified crops | |
Wang, Jinbin1,3; Wang, Yu2,3; Hu, Xiuwen3; Yang, Qianwen3; Chen, Yifan1,3; Jiang, Wei1,3; Liu, Xiaofeng2,3; Liu, Hua1,3; Zeng, Haijuan1,3 | |
刊名 | FOOD CONTROL |
2022-09-01 | |
卷号 | 139 |
关键词 | Genetically modified crops Lateral flow test strip CRISPR Cas12a On-site detection |
ISSN号 | 0956-7135 |
DOI | 10.1016/j.foodcont.2022.109048 |
英文摘要 | Rapid detection method with high sensitivity has been expected in transgenic field screening. In this study, an ultra-sensitive visualization detection procedure based on recombinase polymerase amplification, CRISPR/ Cas12a system, and lateral flow strip technology (RPA-Cas12a-LFB) was developed for the rapid detection of genetically modified (GM) crops. P-CaMV 35 S and T -NOS screening elements, the most widely covered in GM crops, were targeted. The results showed that standard plasmids as low as 10 copies and 0.01% GM crops of Bt11 and MON863 samples can be detected, demonstrating the ultra-sensitivity of the method. The assay was applied to a series of GM crop standards, and the results were consistent with those of existing methods, exhibiting the excellent specificity and feasibility. The analysis can be completed within 40 min, realizing rapid detection. Overall, an accurate, sensitive, time-saving, and instrument-independent detection system was successfully developed, providing a new strategy with ultra-sensitive capability in detection. |
WOS研究方向 | Food Science & Technology |
语种 | 英语 |
出版者 | ELSEVIER SCI LTD |
WOS记录号 | WOS:000799020100004 |
内容类型 | 期刊论文 |
源URL | [http://ir.lut.edu.cn/handle/2XXMBERH/158923] |
专题 | 能源与动力工程学院 生命科学与工程学院 |
作者单位 | 1.Minist Agr & Rural Affairs, Crops Ecol Environm Secur Inspection & Supervis Ct, 2901 Beidi Rd, Shanghai 201106, Peoples R China; 2.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730050, Peoples R China 3.Shanghai Acad Agr Sci, Inst Biotechnol Res, Key Lab Agr Genet & Breeding, 2901 Beidi Rd, Shanghai 201106, Peoples R China; |
推荐引用方式 GB/T 7714 | Wang, Jinbin,Wang, Yu,Hu, Xiuwen,et al. The development of RPA and CRISPR-Cas12a based immunoassay strip for sensitive detection of genetically modified crops[J]. FOOD CONTROL,2022,139. |
APA | Wang, Jinbin.,Wang, Yu.,Hu, Xiuwen.,Yang, Qianwen.,Chen, Yifan.,...&Zeng, Haijuan.(2022).The development of RPA and CRISPR-Cas12a based immunoassay strip for sensitive detection of genetically modified crops.FOOD CONTROL,139. |
MLA | Wang, Jinbin,et al."The development of RPA and CRISPR-Cas12a based immunoassay strip for sensitive detection of genetically modified crops".FOOD CONTROL 139(2022). |
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