Fabrication of polymer functionalized Mn2+-tannic acid coatings on magnetism-responsive nano-microspheres for Immobilized Penicillin G acylase

doi:10.1016/j.colsurfa.2022.129951

	Fabrication of polymer functionalized Mn2+-tannic acid coatings on magnetism-responsive nano-microspheres for Immobilized Penicillin G acylase
	Lu, Siyuan 1,2; Tu, Hongyi 1,2; Liu, Yangdong 1,2; Zhang, Boyuan 1,2; Chen, Zhenbin 1,2
刊名	Colloids and Surfaces A: Physicochemical and Engineering Aspects
	2022-11-20
卷号	653
关键词	Acrylic monomers Catalyst activity Controlled drug delivery Enzyme immobilization Esters Flavonoids Inverse problems Magnetic bubbles Magnetite Synthesis (chemical) Tannins Enzyme concentrations Enzymes activity Glycidyl methacrylate Immobilisation Immobilization conditions Immobilized penicillin G acylase Magnetic polymer microsphere Mn2+ Penicillin G Acylase Target spacing
ISSN号	0927-7757
DOI	10.1016/j.colsurfa.2022.129951
英文摘要	Firstly, Fe3O4 nanoparticles (Fe3O4 NPs) were synthesized by the inverse microemulsion method, and then, Mn2+ was doped in the process of coating tannin(TA) on the surface of Fe3O4 NPs to obtain Mn2+-Fe3O4@PTA NPs. The composite carrier containing Mn2+ could activate the catalytic activity of immobilized Penicillin G acylase (PGA). After the GMA was introduced into the carrier surface by ring-opening reaction, the above preparation carrier was modified by introducing RAFT reagent to obtain Mn2+-Fe3O4@PTA-GMA-g-RAFT NPs. Lastly, glycidyl methacrylate (GMA) and methyl methacrylate (MMA) were employed as the target monomer and the monomer for regulating the spacing between the targets, respectively. Herein, PGA was covalently immobilized on the surface of the magnetic microspheres by reaction of the amino groups of the PGA molecules with the epoxy groups of the magnetic polymer microspheres. Selections of immobilization conditions, including enzyme concentration, pH, temperature, and time of immobilization, were explored. The condition under which enzyme loading capacity (ELC), enzyme activity (EA), and enzyme activity recovery (EAR) of immobilized PGA were maximized as follows: enzyme concentration 2.5 vol%, pH = 8.0, time 20 h, and temperature 37 °C. Under the optimal immobilization conditions, ELC, EA, and EAR of immobilized PGA were 120 mg/g, 31,361 U/g, and 93.7%, respectively. Immobilized PGA remained 90.1% of initial activity and the carrier recovery was 97.8% after 14 successive cycles. These results indicated that the biocatalytic systems with potential use in biomedical application by the influence of polyhydroxy groups, target spacing, and Mn2+. © 2022 Elsevier B.V.
语种	英语
出版者	Elsevier B.V.
内容类型	期刊论文
源URL	[http://ir.lut.edu.cn/handle/2XXMBERH/159739]
专题	材料科学与工程学院
作者单位	1.State Key Laboratory of Advanced progressing and Recycling of Nonferrous Metal Materials, Lanzhou University of Technology, Lanzhou; 730050, China 2.School of Materials Science and Engineering, Lanzhou University of Technology, Lanzhou; 730050, China;
推荐引用方式 GB/T 7714	Lu, Siyuan,Tu, Hongyi,Liu, Yangdong,et al. Fabrication of polymer functionalized Mn2+-tannic acid coatings on magnetism-responsive nano-microspheres for Immobilized Penicillin G acylase[J]. Colloids and Surfaces A: Physicochemical and Engineering Aspects,2022,653.
APA	Lu, Siyuan,Tu, Hongyi,Liu, Yangdong,Zhang, Boyuan,&Chen, Zhenbin.(2022).Fabrication of polymer functionalized Mn2+-tannic acid coatings on magnetism-responsive nano-microspheres for Immobilized Penicillin G acylase.Colloids and Surfaces A: Physicochemical and Engineering Aspects,653.
MLA	Lu, Siyuan,et al."Fabrication of polymer functionalized Mn2+-tannic acid coatings on magnetism-responsive nano-microspheres for Immobilized Penicillin G acylase".Colloids and Surfaces A: Physicochemical and Engineering Aspects 653(2022).