Design and synthesis of thermosensitive block resin as carrier for immobilization of penicillin G acylase

doi:10.1108/PRT-01-2021-0011

	Design and synthesis of thermosensitive block resin as carrier for immobilization of penicillin G acylase
	Tu, Hongyi; Liu, Donglei; Chen, Zhenbin; Liu, Chunli
刊名	PIGMENT & RESIN TECHNOLOGY
	2021-12
关键词	Low critical solution temperature (LCST) Penicillin G acylase (PGA) Segment order Thermosensitive block resin
ISSN号	0369-9420
DOI	10.1108/PRT-01-2021-0011
英文摘要	Purpose Using a reversible addition fragmentation chain transfer reaction, a series of resins were prepared by using N, N-diethyl acrylamide (DEA), poly (ss-hydroxyethyl methacrylate) (PHEMA) as hydrophilic blocks and poly (glycidyl methacrylate) (PGMA) as hydrophobic blocks (and as a target for immobilizing penicillin G acylase [PGA]) and the low critical solution temperature (LCST) of which could be adjusted by changing the segment length of blocks. Design/methodology/approach To make the catalytic conversion temperature of immobilized PGA fallen into the temperature range of the sol state of thermosensitive block resin, a type of thermosensitive block resin, i.e. PDEA-b-PHEMA-b-PGMA (DHGs) was synthesized to immobilize PGA, and the effect of segment order of block resin was investigated on the performance of PGA. Findings Carrier prepared with monomers molar ratio of n(DEA) : n(HEMA): n(GMA) = 100: 49: 36 presented loading capacity (L) and enzyme activity recovery ratio (Ar) of 110 mg/g and 90%, respectively, and a block resin with LCST value of 33 degrees C was essential for keeping higher Ar of PGA. Originality/value PGA has become an important biocatalyst in modern chemistry industry. However, disadvantages include difficulty in separation, poor repeatability and high cost, which limits the scope of PGA applications. The effective method is to immobilize the enzyme to the carrier, which could overcome the disadvantage of free enzyme.
WOS研究方向	Chemistry ; Engineering ; Materials Science
语种	英语
出版者	EMERALD GROUP PUBLISHING LTD
WOS记录号	WOS:000724200700001
内容类型	期刊论文
源URL	[http://ir.lut.edu.cn/handle/2XXMBERH/150024]
专题	材料科学与工程学院
作者单位	Lanzhou Univ Technol, State Key Lab Gansu Adv Nonferrous Met Mat, Lanzhou, Peoples R China; Lanzhou Univ Technol, Sch Mat Sci & Engn, Lanzhou, Peoples R China
推荐引用方式 GB/T 7714	Tu, Hongyi,Liu, Donglei,Chen, Zhenbin,et al. Design and synthesis of thermosensitive block resin as carrier for immobilization of penicillin G acylase[J]. PIGMENT & RESIN TECHNOLOGY,2021.
APA	Tu, Hongyi,Liu, Donglei,Chen, Zhenbin,&Liu, Chunli.(2021).Design and synthesis of thermosensitive block resin as carrier for immobilization of penicillin G acylase.PIGMENT & RESIN TECHNOLOGY.
MLA	Tu, Hongyi,et al."Design and synthesis of thermosensitive block resin as carrier for immobilization of penicillin G acylase".PIGMENT & RESIN TECHNOLOGY (2021).